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Titolo:
Identification by subtractive hybridization of sequences specific for Salmonella enterica serovar Enteritidis
Autore:
Agron, PG; Walker, RL; Kinde, H; Sawyer, SJ; Hayes, DC; Wollard, J; Andersen, GL;
Indirizzi:
Lawrence Livermore Natl Lab, Biol & Biotechnol Res Program, Livermore, CA 94551 USA Lawrence Livermore Natl Lab Livermore CA USA 94551 ivermore, CA 94551 USA Univ Calif Davis, Sch Vet Med, Calif Anim Hlth & Food Safety Lab, Davis Branch, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 ab, Davis Branch, Davis, CA 95616 USA Univ Calif Davis, Sch Vet Med, San Bernardino Branch, Calif Anim Hlth & Food Safety Lab, San Bernardino, CA 92408 USA Univ Calif Davis San Bernardino CA USA 92408 San Bernardino, CA 92408 USA
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 11, volume: 67, anno: 2001,
pagine: 4984 - 4991
SICI:
0099-2240(200111)67:11<4984:IBSHOS>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENVIRONMENTAL SWABS; POULTRY HOUSES; UNITED-STATES; PLASMID; ASSAY; DNA; EPIDEMIOLOGY; TYPHIMURIUM; FIMBRIAE; STRAINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Andersen, GL Lawrence Livermore Natl Lab, Biol & Biotechnol Res Program, 7000 E Ave,L-441, Livermore, CA 94550 USA Lawrence Livermore Natl Lab 7000 EAve,L-441 Livermore CA USA 94550
Citazione:
P.G. Agron et al., "Identification by subtractive hybridization of sequences specific for Salmonella enterica serovar Enteritidis", APPL ENVIR, 67(11), 2001, pp. 4984-4991

Abstract

Salmonella enterica serovar Enteritidis, a major cause of food poisoning, can be transmitted to humans through intact chicken eggs when the contents have not been thoroughly cooked. Infection in chickens is asymptomatic; therefore, simple, sensitive, and specific detection methods are crucial for efforts to limit human exposure. Suppression subtractive hybridization was used to isolate DNA restriction fragments present in Salmonella serovar Enteritidis but absent in other bacteria found in poultry environments. Oligonucleotide primers to candidate regions were used in polymerase chain reactions to test 73 non-Enteritidis S. enterica isolates comprising 34 different serovars, including Dublin and Pullorum, two very close relatives of Enteritidis. A primer pair to one Salmonella difference fragment (termed Sdf I) clearly distinguished serovar Enteritidis from all other serovars tested, while two other primer pairs only identified a few non-Enteritidis strains. These primer pairs were also useful for the detection of a diverse collection of clinical and environmental Salmonella serovar Enteritidis isolates. Inaddition, five bacterial genera commonly found with Salmonella serovar Enteritidis were not detected. By treating total DNA with an exonuclease that degrades sheared chromosomal DNA but not intact circular plasmid DNA, it was shown that Sdf I is located on the chromosome. The Sdf I primers were used to screen a Salmonella serovar Enteritidis genomic library and a unique 4,060-bp region was defined. These results provide a basis for developing a rapid, sensitive, and highly specific detection system for Salmonella serovar Enteritidis and provide sequence information that may be relevant to theunique characteristics of this serovar.

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Documento generato il 14/07/20 alle ore 06:32:33