Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The role of desmin and N-cadherin in effusion cytology - A comparative study using established markers of mesothelial and epithelial cells
Autore:
Davidson, B; Nielsen, S; Christensen, J; Asschenfeldt, P; Berner, A; Risberg, B; Johansen, P;
Indirizzi:
Norwegian Radium Hosp, Dept Pathol, Div Cytol, N-0310 Oslo, Norway Norwegian Radium Hosp Oslo Norway N-0310 Div Cytol, N-0310 Oslo, Norway Aalborg Hosp, Dept Pathol, DK-9000 Aalborg, Denmark Aalborg Hosp AalborgDenmark DK-9000 pt Pathol, DK-9000 Aalborg, Denmark
Titolo Testata:
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
fascicolo: 11, volume: 25, anno: 2001,
pagine: 1405 - 1412
SICI:
0147-5185(200111)25:11<1405:TRODAN>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEROUS EFFUSIONS; MALIGNANT MESOTHELIOMA; DIFFERENTIAL-DIAGNOSIS; PLEURAL MESOTHELIOMA; CARCINOMA-CELLS; LUNG ADENOCARCINOMAS; ADHESION MOLECULE; IN-VIVO; EXPRESSION; IMMUNOHISTOCHEMISTRY;
Keywords:
serous effusions; immunocytochemistry; mesothelial cells; carcinoma cells; sensitivity; specificity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Davidson, B Norwegian Radium Hosp, Dept Pathol, Div Cytol, N-0310 Oslo, Norway Norwegian Radium Hosp Oslo Norway N-0310 N-0310 Oslo, Norway
Citazione:
B. Davidson et al., "The role of desmin and N-cadherin in effusion cytology - A comparative study using established markers of mesothelial and epithelial cells", AM J SURG P, 25(11), 2001, pp. 1405-1412

Abstract

The objective of the present study was to analyze the role of the mesothelial markers desmin and N-cadherin in the diagnostic panel of serous effusions. A total of 181 pleural and peritoneal effusions consisted of 101 cases cytologically diagnosed as malignant (89 carcinomas, 12 mesotheliomas), 78 benign, and 2 inconclusive specimens. All specimens were immunostained using 11 antibodies, against epithelial membrane antigen, BerEP4, carcinoembryonic antigen, E-cadherin, CA 125, N-cadherin, desmin, calretinin, p53, vimentin, and CD45. After evaluation of immunocytochemistry results, 110 specimens were diagnosed as malignant (98 carcinomas, 12 mesotheliomas) and 71 as benign (56 cellular, 15 paucicellular). The presence of desmin was detectedin benign mesothelial cells in 47 of 56 (84%) reactive cellular specimens compared with I of 12 (8%) malignant mesotheliomas and 2 of 98 (2%) carcinomas. N-cadherin was expressed in 48 of 56 (86%) reactive cases, 12 of 12 (100%) mesotheliomas, and 47 of 98 (48%) carcinomas, In carcinomas, N-cadherin expression was most often seen in ovarian carcinoma but was also found inother carcinomas. Calretinin, an established marker of mesothelial cells, was detected in 52 of 56 (93%) reactive specimens, 11 of 12 (93%) mesotheliomas, and 3 of 98 (3%) carcinomas. Evaluation of staining results led to reclassification of six malignant specimens as benign, whereas 17 cases diagnosed as benign and the two diagnosed as inconclusive were classified as malignant. In conclusion, desmin appears to be a promising marker for the distinction between reactive mesothelium and malignant epithelial cells in terms of both specificity and sensitivity, and its complementary use with calretinin is recommended. Unlike calretinin, it may also prove valuable for thedistinction between benign and malignant mesothelial cells. N-cadherin does not have a role in the distinction between mesothelial and epithelial cells. However, it may prove useful in the characterization of carcinomas of unknown origin. As has previously been shown, a significant number of diagnoses that are based on morphologic examination alone are modified after the use of a broad antibody panel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:50:18