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Titolo:
GFP-centrin as a marker for centriole dynamics in the human breast cancer cell line MCF-7
Autore:
DAssoro, AB; Stivala, F; Barrett, S; Ferrigno, G; Salisbury, JL;
Indirizzi:
Mayo Clin & Mayo Fdn, Tumor Biol Program, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 gram, Rochester, MN 55905 USA
Titolo Testata:
ADVANCES IN MICROANATOMY OF CELLS AND TISSUES, BIOPHYSICAL AND BIOCHEMICALCORRELATES
, volume: 7, anno: 2001,
pagine: 103 - 110
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN-FLUORESCENT PROTEIN; CENTROSOME DUPLICATION; HYPERAMPLIFICATION; OVEREXPRESSION; ORGANIZATION; REPRODUCTION; INSTABILITY; TUMORS; P53;
Keywords:
cancer; cell cycle; centrosomes; mitosis; aneuploidy; genomic instability;
Tipo documento:
Article
Natura:
Collana
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Salisbury, JL Mayo Clin & Mayo Fdn, Tumor Biol Program, 200 1st St SW, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn 200 1st St SW Rochester MN USA 55905 SA
Citazione:
A.B. D'Assoro et al., "GFP-centrin as a marker for centriole dynamics in the human breast cancer cell line MCF-7", MA MA SY SE, 7, 2001, pp. 103-110

Abstract

Centrosome duplication plays an important role in genomic stability through bipolar spindle formation and equal chromosome segregation during mitosis. Defects in centrosome duplication and centrosome amplification correlate with aggressive tumors and aneuploidy. Cyclin-dependent cell cycle regulators play a key role in signaling centrosome duplication and the tumor suppressor genes p53, BRCA1 and BRCA2 are suspected to function at mitotic checkpoints that monitor centrosome duplication. The relationship between loss ofhormone dependence in breast cancer, and signaling of centrosome duplication in tumor progression is not known, We have developed a MCF-7 cell line expressing GFP-centrin that allows direct visualization of centriole duplication during the cell cycle in living cells. GFP-centrin is expressed and selectively incorporated into the structure of both centrioles making them clearly visible in living cells. Our studies demonstrate three important aspects of recombinant GFP-centrin incorporation into centrioles. 1) GFP-centrin transfected cells grow norm-ally in culture and show no adverse effect associated with GFP-centrin expression; 2) newly duplicated centrioles incorporate centrin during their genesis; and 3) GFP-centrin incorporation into centrioles does not grossly affect cell cycle progression, or centrosome function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 04:04:44