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Titolo:
Intestinal expressions of eNOSmRNA and iNOSmRNA in rats with acute liver failure
Autore:
Qin, JM; Zhang, YD;
Indirizzi:
Hunan Med Univ, Xiangya Hosp, Dept Hepatobiliary & Enter Surg, Changsha 410008, Hunan Province, Peoples R China Hunan Med Univ Changsha Hunan Province Peoples R China 410008 les R China
Titolo Testata:
WORLD JOURNAL OF GASTROENTEROLOGY
fascicolo: 5, volume: 7, anno: 2001,
pagine: 652 - 656
SICI:
1007-9327(200110)7:5<652:IEOEAI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; HEPATIC ISCHEMIA-REPERFUSION; BACTERIAL TRANSLOCATION; HEMORRHAGIC-SHOCK; BLOOD-FLOW; ENDOTOXIN; TRANSIT; MICE; INHIBITION; APOPTOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Qin, JM Mil Med Coll 2, Eastern Hepatobiliary Surg Hosp, Biol Signal Transduct Ctr, Shanghai 200438, Peoples R China Mil Med Coll 2 Shanghai Peoples R China 200438 , Peoples R China
Citazione:
J.M. Qin e Y.D. Zhang, "Intestinal expressions of eNOSmRNA and iNOSmRNA in rats with acute liver failure", WORLD J GAS, 7(5), 2001, pp. 652-656

Abstract

AIM To observe the gene expression change of eNOSmRNA and iNOSmRNA in the small and large intestines with acute liver failure (ALF), and to reveal the biological function of NO on the pathogeniesis of ALF and multiple organsdysfunction at the molecular level. METHODS Sixty male Wistar rats were selected, weighing from 250 g to 350 g, and divided into 5 groups randomly: SO, ALF (6 h, 12 h), L-Arg, L-NAME, L-Arg and L-NAME, each group with 10 rats. The dose of L-Arg was 300 mg . kg(-1), and L-NAME was 30 mg . kg(-1), the reagents diluted by normal saline were injected through tail vein 30 minutes pre- and post-operation. The rats in the ALF group were respectively sacrificed postoperatively at 6 h, 12 h, and the rats in the other groups were sacrificed postoperatively at 6 h. The tissues of small and large intestines were harvested in 4% paraforaldehyde containing the reagent of DEPC and fixed at 6 h, embedded in paraffin,and 4 pm section was cut. The expression of eNOSmRNA and iNOSmRNA in thesetissues was determined with in situ hybridization, and analyzed with the imaging analysis system of CMM-3 and SPSS statistical software. RESULTS The expression of eNOSmRNA in the large intestine and iNOSmRNA in the small and large intestines increased significantly at 6 h after ALF, but the expression of iNOSmRNA in the small and large intestines reduced notably at 12 h after ALF (P <0.05); the expression of eNOSmRNA in the large intestine and iNOSmRNA in the small and large intestines decreased significantly with the reagents of L-Arg at 6 h ALF, but the expression of eNOSmRNA and iNOSmRNA in the small and large intestines decreased totally with the reagents of L-NAME or association with L-Arg 6 h ALF. CONCLUSION The expression of eNOSmRNA in the large intestine increased notably at the early stage of ALF, No induced by the enzyme of eNOS from the transplantation of eNOSmRNA can protect the function of the large intestine,the high expression of iNOSmRNA is involved in the damaged function of thesmall and large intestines. NO precursor can reduce the expression of iNOSmRNA in the small and large intestines and the damage to intestines; NOS inhibitor or association with NO precursor can totally lower the expression of eNOSmRNA and iNOSmRNA in the small and large intestines, it cannot notably influence the NOS inhibitor in the gene expression of eNOSmRNA and iNOSmRNA to supply the additional NO precursor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 20:13:17