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Titolo:
Effects of hypoxia, hyperoxia on the regulation of expression and activityof matrix metalloproteinase-2 in hepatic stellate cells
Autore:
Chen, PS; Zhai, WR; Zhou, XM; Zhang, JS; Zhang, YE; Ling, YQ; Gu, YH;
Indirizzi:
Fudan Univ, Sch Basic Med Sci, Dept Pathol, Shanghai 200032, Peoples R China Fudan Univ Shanghai Peoples R China 200032 nghai 200032, Peoples R China Nanjing Railway Med Coll, Nanjing, Peoples R China Nanjing Railway Med Coll Nanjing Peoples R China njing, Peoples R China Inst Canc Res, Shanghai 200032, Peoples R China Inst Canc Res Shanghai Peoples R China 200032 ai 200032, Peoples R China
Titolo Testata:
WORLD JOURNAL OF GASTROENTEROLOGY
fascicolo: 5, volume: 7, anno: 2001,
pagine: 647 - 651
SICI:
1007-9327(200110)7:5<647:EOHHOT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROTIC HUMAN LIVER; TISSUE INHIBITOR; RAT-LIVER; ENDOTHELIAL-CELLS; ACTIVATION; FIBROSIS; HEPATOCYTES; FIBROGENESIS; MECHANISMS; OXYGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Zhai, WR Fudan Univ, Sch Basic Med Sci, Dept Pathol, 138 Yixueyuan Rd, Shanghai 200032, Peoples R China Fudan Univ 138 Yixueyuan Rd Shanghai PeoplesR China 200032 hina
Citazione:
P.S. Chen et al., "Effects of hypoxia, hyperoxia on the regulation of expression and activityof matrix metalloproteinase-2 in hepatic stellate cells", WORLD J GAS, 7(5), 2001, pp. 647-651

Abstract

AIM To study the effects of hypoxia! hyperoxia on the regulation of expression and activity of matrix metalloproteinase-2 (MMP-2) in hepatic stellatecells (HSC). METHODS The expressions of MMP-2, tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) and membrane type matrix metalloproteinase-1 (MT1-MMP) in cultured rat HSC were detected by immunocytochemistry (ICC) and in situ hybridization (ISH). The contents of MMP-2 and TIMP-2 in culture supernatant were detected with ELISA and the activity of MMP-2 in supernatant was revealed by zymography. RESULTS In the situation of hypoxia for 12 h, the expression of MMP-2 protein was enhanced ( hypoxia group positive indexes: 5.7 +/- 2.0, n = 10; control: 3.2 +/- 1.0, n = 7; P < 0.05), while TIMP-2 protein was decreased in HSC (hypoxia group positive indexes: 2.5 +/- 0.7, n = 10; control: 3.6 +/- 1.0, n = 7; P <0.05), and the activity (total A) of MMP-2 in supernatant declined obviously (hypoxia group. 7.334 +/- 1.922, n = 9; control: 17.277 +/- 7.424, n = 11; P <0.01). Compared the varied duration of hypoxia, the changes of expressions including mRNA and protein level as well as activity ofMMP-2 were most notable in 6 h group. The highest value (A(hypoxia)-A(control)) Of the protein and the most intense signal of mRNA were in the periodof hypoxia for 6 h, along with the lowest activity of MMP-2. In the situation of hyperoxia for 12 h, the contents (A(450)) of MMP-2 and MMP-2 in supernatant were both higher than those in the control, especially the MMP-2 (hyperoxia group: 0.0499 +/- 0.0144, n = 16; control: 0.0219 +/- 0.0098, n = 14; P <0.01), and so was the activity of MMP-2 (hyperoxia group: 5.252 +/- 0.771, n = 14; control: 4.304 +/- 1.083, n = 12; P <0.05), and the expression of MTI-MMP was increased. CONCLUSION HSC is sensitive to the oxygen, hypoxia enhances the expressionof MMP-2 and the effect is more marked at the early stage; hyperoxia mainly raises the activity of MMP-2.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 09:11:28