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Titolo:
Comparison of dose-responses of contact allergens using the guinea pig maximization test and the local lymph node assay
Autore:
van Och, FMM; Vandebriel, RJ; Prinsen, MK; De Jong, WH; Slob, W; van Loveren, H;
Indirizzi:
Natl Inst Publ Hlth & Environm, RIVM, NL-3720 BA Bilthoven, Netherlands Natl Inst Publ Hlth & Environm Bilthoven Netherlands NL-3720 BA herlands TNO, Nutr & Food Res Inst, NL-3700 AJ Zeist, Netherlands TNO Zeist Netherlands NL-3700 AJ Res Inst, NL-3700 AJ Zeist, Netherlands
Titolo Testata:
TOXICOLOGY
fascicolo: 3, volume: 167, anno: 2001,
pagine: 207 - 215
SICI:
0300-483X(20011030)167:3<207:CODOCA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHEMICALS; POTENCY;
Keywords:
contact allergens; DEA; dose-response; guinea pig maximization test; local lymph node assay; sensitizing potency; TMTD; ZDMC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: van Loveren, H Natl Inst Publ Hlth & Environm, RIVM, POB 1, NL-3720 BA Bilthoven, Netherlands Natl Inst Publ Hlth & Environm POB 1 Bilthoven Netherlands NL-3720 BA
Citazione:
F.M.M. van Och et al., "Comparison of dose-responses of contact allergens using the guinea pig maximization test and the local lymph node assay", TOXICOLOGY, 167(3), 2001, pp. 207-215

Abstract

The guinea pig maximization test (GPMT) has been used as a method for the prediction of skin sensitizing potential for over 30 years. Besides hazard identification, risk assessment of sensitizing chemicals requires the assessment of potency. For the determination of potency based on lowest effective dose levels, dose-response studies are required. In the standard GPMT a single concentration is used for intracutaneous and topical induction and the assay provides a qualitative assessment of allergenicity. This paper presents data derived from quantitative evaluation of the sensitizing potency of chemicals in the GPMT, based on multiple concentrations. We performed theGPMT in accordance with the original procedure of Magnusson and Kligman; and included in this procedure a range of intradermal and topical concentrations for induction. Three allergens with different sensitizing potencies, diethylamine (DEA), tetramethyl thiuram disulfide (TMTD) and zinc dimethyl dithiocarbamate (ZDMC) were tested. The data obtained with this test procedure were compared to data we previously obtained using the local lymph node assay (LLNA). Both the GPMT and the LLNA showed dose response relationshipsfor the three chemicals tested. For the chemicals tested, both tests differed in the relative potencies based on benchmark concentrations. While bothtests ranked DEA as the least potent allergen, the GPMT ranked ZDMC more potent than TMTD, the reverse being found in the LLNA. The nature of the data provided in the LLNA makes it likely that benchmarks as defined with thistest are more reliable than that defined in the GPMT. However, further validation with human data is necessary. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:39:07