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Titolo:
Genes upregulated in lead-resistant glioma cells reveal possible targets for lead-induced developmental neurotoxicity
Autore:
Li, P; Rossman, TG;
Indirizzi:
NYU, Nelson Inst Environm Med, Sch Med, Tuxedo Pk, NY 10987 USA NYU Tuxedo Pk NY USA 10987 Environm Med, Sch Med, Tuxedo Pk, NY 10987 USA
Titolo Testata:
TOXICOLOGICAL SCIENCES
fascicolo: 1, volume: 64, anno: 2001,
pagine: 90 - 99
SICI:
1096-6080(200111)64:1<90:GUILGC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; ENDOTHELIAL GROWTH-FACTOR; CENTRAL-NERVOUS-SYSTEM; HEPARAN-SULFATE; STRUCTURAL REQUIREMENTS; SEMAPHORIN-III; INORGANIC LEAD; THROMBOSPONDIN-1; NEUROPILIN-1; EXPRESSION;
Keywords:
lead toxicity; glial cells; thrombospondin-1; heparin sulfate 6-sulfotransferase; neuropilin-1; PbR11 cells; C6 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Rossman, TG NYU, Nelson Inst Environm Med, Sch Med, 57 Old Forge Rd, Tuxedo Pk, NY 10987 USA NYU 57 Old Forge Rd Tuxedo Pk NY USA 10987 do Pk, NY 10987 USA
Citazione:
P. Li e T.G. Rossman, "Genes upregulated in lead-resistant glioma cells reveal possible targets for lead-induced developmental neurotoxicity", TOXICOL SCI, 64(1), 2001, pp. 90-99

Abstract

Identifying genes upregulated in lead-resistant cells should give insight into lead toxicity and cellular protective mechanisms and may also result in identification of proteins that may be useful as biomarkers. Glial cells are thought to protect neurons against heavy metals. Rat glioma C6 cells share many properties of normal glial cells. To identify and analyze genes upregulated in a lead-resistant variant, PbR11, suppression subtractive hybridization (SSH) between mRNAs of wild-type and PbR11 cells was performed. Sequencing and database searches identified three genes, thrombospondin-1, heparin sulfate 6-sulfotransferase, and neuropilin-1, which play important roles in angiogenesis and axon growth during development. Two genes, HSP90 and UBA3, are involved in the ubiquitin-proteosome system. One gene was identified as that of a rat endogenous retrovirus and another, 2C9, is a transcript expressed in fos-transformed cells. PbR11 also overexpresses c-fos. Expression of these genes and effects of short-term lead exposure (24 h, up to600 muM) on their expression in C6 cells was examined. The rat endogenous retrovirus and 2C9 are expressed only in PbR11 cells, and show no expression, either constitutive or lead-induced, in wild-type C6 cells. HSP90 is expressed at low level constitutively in C6 cells, but can be induced in a dose-dependent manner by lead. In contrast, thrombospondin-1 is repressed in adose-dependent manner by lead. The other genes (HS6ST, neuropilin, and UBA3) show low constitutive expression and are neither upregulated nor downregulated by exposure to lead. We suggest that neuropilin-1, heparin sulfate 6-sulfotransferase, and thrombospondin-1 may be important targets for lead-induced developmental neurotoxicity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 05:12:21