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Titolo:
Zinc-metallothionein levels are correlated with enhanced glucocorticoid responsiveness in mouse cells exposed to ZnCl2, HgCl2, and heat shock
Autore:
DeMoor, JM; Kennette, WA; Collins, OM; Koropatnick, J;
Indirizzi:
London Reg Canc Ctr, London, ON N6A 4L6, Canada London Reg Canc Ctr London ON Canada N6A 4L6 , London, ON N6A 4L6, Canada Univ Western Ontario, Dept Microbiol & Immunol, London, ON, Canada Univ Western Ontario London ON Canada biol & Immunol, London, ON, Canada Univ Western Ontario, Dept Oncol, London, ON, Canada Univ Western OntarioLondon ON Canada io, Dept Oncol, London, ON, Canada Univ Western Ontario, Dept Pharmacol & Toxicol, London, ON, Canada Univ Western Ontario London ON Canada acol & Toxicol, London, ON, Canada Univ Western Ontario, Dept Pathol, London, ON, Canada Univ Western Ontario London ON Canada o, Dept Pathol, London, ON, Canada
Titolo Testata:
TOXICOLOGICAL SCIENCES
fascicolo: 1, volume: 64, anno: 2001,
pagine: 67 - 76
SICI:
1096-6080(200111)64:1<67:ZLACWE>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE-EXPRESSION; DNA-BINDING DOMAIN; TYROSINE AMINOTRANSFERASE; MOLECULAR CHAPERONES; DOWN-REGULATION; RECEPTOR; TRANSCRIPTION; LIVER; RAT; THIONEIN;
Keywords:
metallothionein; metals; zinc; glucocorticoid receptor; heat shock;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Koropatnick, J London Reg Canc Ctr, 790 Commissioners Rd E, London, ON N6A4L6, Canada London Reg Canc Ctr 790 Commissioners Rd E London ON Canada N6A 4L6
Citazione:
J.M. DeMoor et al., "Zinc-metallothionein levels are correlated with enhanced glucocorticoid responsiveness in mouse cells exposed to ZnCl2, HgCl2, and heat shock", TOXICOL SCI, 64(1), 2001, pp. 67-76

Abstract

Metallothioneins (MTs) are the major low molecular weight, zinc-binding proteins in mammalian cells. It has been hypothesized that they play a role in the function of zinc-dependent signal transduction proteins and transcription factors. We investigated the capacity of zinc and other metal ions andconditions to increase both Zn-associated NIT levels and the receptivenessof cells to transcriptional activation mediated by the zinc-dependent glucocorticoid receptor (GR). We studied, in a GR-responsive mouse mammary-tumor cell line, the ability of dexamethasone (DEX) to stimulate transcription of a chloramphenicol acetyltransferase (CAT) gene controlled by a mouse mammary-tumor virus promoter. In cells pretreated with 20 to 100 muM ZnCl2, DEX-induced CAT activity correlated with zinc-induced NIT levels. However, 0.05 to 0.5 muM CdCl2 had no effect on CAT activity, despite an increase in Cd-associated MT. Copper-associated MT was detected in cells treated with 20muM CuCl2, but there was no change in the level of Zn-MT, nor was CAT activity altered in cells exposed to 5 to 20 muM CuCl2. These results may reflect a functional difference between zinc-associated MT, and MT associated with other metals. Significantly more CAT activity was observed in both beat-shocked cells and in cells exposed to 40 or 50 nM HgCl2. Although absolute amounts of MT were unchanged by these two treatments, a higher percentage of total cellular zinc was associated with the MT protein fractions after treatment. Changes in GR levels could not account for variations in CAT activity. These data indicate that hormonal signalling can be altered by exposure to metal salts and heat shock, and the effect is correlated with the level of Zn-MT.

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Documento generato il 26/01/20 alle ore 10:11:36