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Titolo:
Genetic mapping of gray leaf spot (GLS) resistance genes in maize
Autore:
Lehmensiek, A; Esterhuizen, AM; van Staden, D; Nelson, SW; Retief, AE;
Indirizzi:
Univ Stellenbosch, Dept Genet, ZA-7602 Matieland, South Africa Univ Stellenbosch Matieland South Africa ZA-7602 Matieland, South Africa Sensako, ZA-0250 Brits, South Africa Sensako Brits South Africa ZA-0250Sensako, ZA-0250 Brits, South Africa
Titolo Testata:
THEORETICAL AND APPLIED GENETICS
fascicolo: 5, volume: 103, anno: 2001,
pagine: 797 - 803
SICI:
0040-5752(200110)103:5<797:GMOGLS>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
QUANTITATIVE TRAIT LOCI; BULKED SEGREGANT ANALYSIS; MOLECULAR MARKERS; MENDELIAN FACTORS; INBRED LINES; LINKAGE MAP; IDENTIFICATION; DISEASE; CROSSES; RAPD;
Keywords:
gray leaf spot; maize; bulked segregant analysis; AFLPs; QTL analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Retief, AE Univ Stellenbosch, Dept Genet, Private Bag 11, ZA-7602 Matieland, South Africa Univ Stellenbosch Private Bag 11 Matieland South Africa ZA-7602
Citazione:
A. Lehmensiek et al., "Genetic mapping of gray leaf spot (GLS) resistance genes in maize", THEOR A GEN, 103(5), 2001, pp. 797-803

Abstract

Bulked segregant analysis was used to identify amplified fragment length polymorphism markers (AFLPs) linked to quantitative trait loci (QTLs) involved in the resistance to gray leaf spot (GLS) in maize. By using ten AFLP primer combinations 11 polymorphic markers were identified and converted to sequence-specific PCR markers. Five of the 11 converted AFLPs were linked tothree GLS resistance QTLs. The markers were mapped to maize chromosomes 1,3 and 5 using existing linkage maps of two commercially available recombinant inbred-line populations. Converted restriction fragment length polymorphism markers and microsatellite markers were used to obtain a more-precise localization for the detected QTLs. The QTL on chromosome 1 was localized in bin 1.05/06 and had a LOD score of 21. A variance of 37% was explained bythe QTL. Two peaks were visible on chromosome 5, one was localized in bin 5.03/04 and the other in bin 5.05/06. Both peaks had a LOD score of 5, and 11% of the variance was explained by the QTLs. A variance of 8-10% was explained by the QTL on chromosome 3 (bin 3.04). The consistency of the QTLs was tested across two F-2 populations planted in consecutive years.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:31:08