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Titolo:
Virus resistance in transgenic sweetpotato [Ipomoea batatas L. (Lam)] expressing the coat protein gene of sweet potato feathery mottle virus
Autore:
Okada, Y; Saito, A; Nishiguchi, M; Kimura, T; Mori, M; Hanada, K; Sakai, J; Miyazaki, C; Matsuda, Y; Murata, T;
Indirizzi:
Kyushu Tokai Univ, Kumamoto 8691404, Japan Kyushu Tokai Univ Kumamoto Japan 8691404 i Univ, Kumamoto 8691404, Japan Kyushu Natl Agr Expt Stn, Kumamoto 8611192, Japan Kyushu Natl Agr Expt Stn Kumamoto Japan 8611192 Kumamoto 8611192, Japan Natl Inst Agrobiol Resources, Tsukuba, Ibaraki 3058602, Japan Natl Inst Agrobiol Resources Tsukuba Ibaraki Japan 3058602 3058602, Japan Japan Sci & Technol Corp, Nagasaki Lab, Nagasaki 8560026, Japan Japan Sci & Technol Corp Nagasaki Japan 8560026 Nagasaki 8560026, Japan
Titolo Testata:
THEORETICAL AND APPLIED GENETICS
fascicolo: 5, volume: 103, anno: 2001,
pagine: 743 - 751
SICI:
0040-5752(200110)103:5<743:VRITS[>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLANT-REGENERATION; L LAM; AGROBACTERIUM-TUMEFACIENS; NUCLEOTIDE-SEQUENCE; GENOMIC RNA; TRANSFORMATION; PROTOPLASTS; MESOPHYLL;
Keywords:
sweet potato feathery mottle virus; coat protein gene; electroporation; virus resistance; transgenic sweetpotato;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Okada, Y Sapporo Breweries Ltd, Plant Bioengn Res Labs, Kizaki,Nitta, Gunma 3700393, Japan Sapporo Breweries Ltd Kizaki,Nitta Gunma Japan 3700393 93, Japan
Citazione:
Y. Okada et al., "Virus resistance in transgenic sweetpotato [Ipomoea batatas L. (Lam)] expressing the coat protein gene of sweet potato feathery mottle virus", THEOR A GEN, 103(5), 2001, pp. 743-751

Abstract

One of the most-serious diseases of sweet potato [Ipomoea batatas (L. ) Lam] is russet crack disease caused by sweet potato feathery mottle virus (SPFMV). We constructed an expression vector carrying the coat protein (CP) andhygromycin phosphotransferase (hpt) genes driven by cauliflower mosaic virus 35 S promoters. Accordingly, we introduced the expression vector into sweet potato variety Chikei 682-11 by the electroporation method. Among 449 calli obtained after antibiotic selection, 19 plants from seven independent calli grew to form adventitious shoots. Three transgenic lines were obtained from independent calli, based on analysis of the CP and hpt genes. The transcription and translation of the CP gene were shown in these transgenic lines by Northern- and Western-blot analyses. To assay the virus resistance of the transgenic lines, each line was vegetatively propagated and then grafted with morning glory (Ipomoea nil) that had been infected with SPFMV-S. A PAS-ELISA assay with polyclonal and serum of the CP demonstrated that virus accumulation 3 months after grafting with the infected morning glory wassuppressed in the transgenic lines as compared with non-transgenic ones. These transgenic lines were shown to be highly resistant not only to primarybut also to secondary infection by SPFMV-S. Thus we concluded that the three transgenic lines with the CP gene of SPFMV-S can be used for coat protein-mediated resistance to the virus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 16:00:22