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Titolo:
GABA/progesterone-induced polyphosphoinositide (PPI) breakdown and its role in the acrosome reaction of guinea pig spermatozoa in vitro
Autore:
Yuan, YY; Mao, LZ; Shi, QX; Roldan, ERS; Chen, WY; Yu, SQ; Zhuang, YZ; Xu, SF;
Indirizzi:
Zhejiang Acad Med Sci, Hangzhou 310013, Peoples R China Zhejiang Acad Med Sci Hangzhou Peoples R China 310013 3, Peoples R China CSIC, Natl Museum Nat Sci, Madrid 28006, Spain CSIC Madrid Spain 28006CSIC, Natl Museum Nat Sci, Madrid 28006, Spain
Titolo Testata:
SCIENCE IN CHINA SERIES C-LIFE SCIENCES
fascicolo: 4, volume: 44, anno: 2001,
pagine: 345 - 355
SICI:
1006-9305(200108)44:4<345:GP(BAI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC-ACID; PROGESTERONE; EXOCYTOSIS; RECEPTOR; PROTEIN; INFLUX;
Keywords:
GABA; progesterone; PIP2; PIP; sperm; PA; acrosome reaction; signal transduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Shi, QX Zhejiang Acad Med Sci, Hangzhou 310013, Peoples R China Zhejiang Acad Med Sci Hangzhou Peoples R China 310013 es R China
Citazione:
Y.Y. Yuan et al., "GABA/progesterone-induced polyphosphoinositide (PPI) breakdown and its role in the acrosome reaction of guinea pig spermatozoa in vitro", SCI CHINA C, 44(4), 2001, pp. 345-355

Abstract

To investigate whether GABA/progesterone (P-4) stimulates PPI breakdown and its role in the acrosome reaction (AR), spermatozoa of guinea pig were preincubated in MCM-LCa2+ for 5.5 h and then labeled with [P-32]pi for 1 h. Samples were washed through a three-step gradient Percoll, adjusted to 5 X 10(7) cells/mL and exposed to 2 mmol/L Ca2+, 5 mu mol/L GABA, 10 mu mol/L P-4 and other agents. Lipids were separated by t.l.c. and radioactivity in spots determined by scintillation counting. The AR was assessed by phase-contrast microscopy. The results showed that (1) when spermatozoa were treated with GABA, P-32-label diminished rapidly in phosphatidylinositol 4, 5-bisphosphate (PIP2), phosphatidylinositol 4-phosphate (PIP), and increased in phosphatidic acid (PA). The loss of label from PPI was almost completed by 10min. The time-course of the AR was much slower than PPI when spermatozoa reached a maximal response by 15 min; (ii) the pattern of PPI hydrolysis andstimulation of AR was similar for the three agonists tested; their potencyfollowed the order A23187 > progesterone greater than or equal to GABA; (iii) GABA-induced PIP2 hydrolysis and rise in PA and the AR were prevented by inclusion of 10 mmol/L neomycin; (iv) the loss of PIP2 labeling and the increase in PA labeling abolished when spermatozoa were exposed to EGTA or Ca2+ channel blocker. These results indicate that GABA or P-4-induced PPI breakdown is an important and essential event in the series of changes to membrane fusion during the AR of guinea pig spermatozoa and this effect is mediated via calcium by activation of phosphatidylinositol-specific phospholipase C.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 22:03:22