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Titolo:
Low levels of nitric oxide (NO) in systemic sclerosis: inducible NO synthase production is decreased in cultured peripheral blood monocyte/macrophagecells
Autore:
Allanore, Y; Borderie, D; Hilliquin, P; Hernvann, A; Levacher, M; Lemarechal, H; Ekindjian, OG; Kahan, A;
Indirizzi:
Univ Paris 05, Hop Cochin, Dept Rheumatol A, F-75014 Paris, France Univ Paris 05 Paris France F-75014 pt Rheumatol A, F-75014 Paris, France Univ Paris 05, Hop Cochin, Dept Biochem A, F-75014 Paris, France Univ Paris 05 Paris France F-75014 Dept Biochem A, F-75014 Paris, France Univ Paris 05, Hop Cochin, Dept Pharmacol, F-75014 Paris, France Univ Paris 05 Paris France F-75014 Dept Pharmacol, F-75014 Paris, France
Titolo Testata:
RHEUMATOLOGY
fascicolo: 10, volume: 40, anno: 2001,
pagine: 1089 - 1096
SICI:
1462-0324(200110)40:10<1089:LLONO(>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
RHEUMATOID-ARTHRITIS; RAYNAUDS-PHENOMENON; CYTOKINE PRODUCTION; MESSENGER-RNA; SCLERODERMA; MONONUCLEAR; ENDOTHELIUM; MACROPHAGES; EXPRESSION; INTERFERON;
Keywords:
nitric oxide; mononuclear cells; systemic sclerosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Kahan, A Univ Paris 05, Hop Cochin, Dept Rheumatol A, 27 Rue Faubourg St Jacques, F-75014 Paris, France Univ Paris 05 27 Rue Faubourg St Jacques Paris France F-75014 ce
Citazione:
Y. Allanore et al., "Low levels of nitric oxide (NO) in systemic sclerosis: inducible NO synthase production is decreased in cultured peripheral blood monocyte/macrophagecells", RHEUMATOLOG, 40(10), 2001, pp. 1089-1096

Abstract

Objective. To investigate nitric oxide (NO) production and inducible NO synthase expression by cultured peripheral blood mononuclear cells (PBMC) in patients with systemic sclerosis (SSc). Methods. Eighteen patients with SSc were compared with two control groups:16 patients with rheumatoid arthritis (RA) and 23 patients with mechanicalsciatica. Nitrate was determined by fluorimetry in plasma and by spectrophotometry in supernatants. Inducible NO synthase (iNOS) was detected in cultured PBMC by immunofluorescence, immunoblotting and flow cytometry With or without treatment of the cells with interleukin (IL) 1 beta + tumour necrosis factor alpha (TNF-alpha), IL-4 or interferon gamma (IFN-gamma) from day 1 to day 5. Results. NO metabolite concentrations were lower in SSc patients (mean +/-S.E.M.. 34.3 +/- 2.63 mu mol/l) than in RA (48.3 +/- 2.82 mu mol/l; P < 0.02) and sciatica (43.3 +/- 5.24 <mu>mol/l; P < 0.03.) patients. iNOS was detected in cultured monocytes in all three groups but induction occurred on day 1 in RA, day 2 in sciatica and only on day 3 in SSc, whatever the Stimulus. Conclusions. The concentrations of NO metabolites are decreased in SSc patients and the metabolism of these compounds in PBMC is altered. Low levels of NO, a vasodilator, may be involved in vasospasm, which is critical in SSc. This may have therapeutic implications.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 01:26:12