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Titolo:
Contribution of proteomics to tumor immunology
Autore:
Le Naour, F;
Indirizzi:
Hop Paul Brousse, INSERM U268, F-94807 Villejuif, France Hop Paul BrousseVillejuif France F-94807 268, F-94807 Villejuif, France
Titolo Testata:
PROTEOMICS
fascicolo: 10, volume: 1, anno: 2001,
pagine: 1295 - 1302
SICI:
1615-9853(200110)1:10<1295:COPTTI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
DENDRITIC CELLS; CANCER-IMMUNOTHERAPY; T-CELLS; ANTIGENS; IDENTIFICATION; CALRETICULIN; LUNG; GENE; LYMPHOCYTES; CARCINOMA;
Keywords:
tumor antigen; autoantibodies; gene expression profiling; dendritic cells; review;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Le Naour, F Hop Paul Brousse, INSERM U268, F-94807 Villejuif, France Hop Paul Brousse Villejuif France F-94807 Villejuif, France
Citazione:
F. Le Naour, "Contribution of proteomics to tumor immunology", PROTEOMICS, 1(10), 2001, pp. 1295-1302

Abstract

In the postgenome era, the global analysis of gene and protein expression is allowed at RNA and protein level by microarrays and proteomics. The application of these complementary approaches provides new opportunities for tumor immunology investigation. Indeed, applied to the study at the molecularlevel of the differentiation and maturation of dendritic cells which are the most potent antigen presenting cells, microarray analysis has identifiedimportant changes in a large number of genes, whereas the proteomic analysis provided information that could not be obtained at the RNA level, such as the separation of different isoforms and the characterization of posttranslational modifications. On the other hand, proteomics allows serological screening of tumor antigens. Indeed, two-dimensional (2-D) polyacrylamide gel electrophoresis allows simultaneously separation several thousand individual proteins from tumor tissue or tumor cell lines. Proteins eliciting humoral response in cancer are identified by 2-D Western blot using cancer patient sera, followed by mass spectrometry analysis and database search. Applied to different types of cancer, the proteome based approach has allowed usto define several tumor antigens. The common occurrence of autoantibodies to certain of these proteins in different cancers may be useful in cancer screening and diagnosis as well as for immunotherapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 04:22:02