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Titolo:
Protein based microarrays: A tool for probing the proteome of cancer cellsand tissues
Autore:
Madoz-Gurpide, J; Wang, H; Misek, DE; Brichory, F; Hanash, SM;
Indirizzi:
Univ Michigan, Sch Med, Dept Pediat, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 Dept Pediat, Ann Arbor, MI 48109 USA
Titolo Testata:
PROTEOMICS
fascicolo: 10, volume: 1, anno: 2001,
pagine: 1279 - 1287
SICI:
1615-9853(200110)1:10<1279:PBMATF>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION PATTERNS; ARRAYS; IMMOBILIZATION; IMMUNOASSAY; SCALE; CHIPS; GOLD;
Keywords:
microarray technology; lung adenocarcinorna cell lysates; rotofor fractions;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Hanash, SM Univ Michigan, Sch Med, Dept Pediat, 1150 W Med Ctr Dr,A520 MSRB 1,Box 0656, Ann Arbor, MI 48109 USA Univ Michigan 1150 W Med Ctr Dr,A520 MSRB 1,Box 0656 Ann Arbor MI USA 48109
Citazione:
J. Madoz-Gurpide et al., "Protein based microarrays: A tool for probing the proteome of cancer cellsand tissues", PROTEOMICS, 1(10), 2001, pp. 1279-1287

Abstract

A novel proteomic approach for probing cell and tissue proteome, which combines liquid phase protein separations with microarray technology has been developed. Proteins in cell and tissue lysates or in cellular subfractions are separated using any one of a number of separation modes which may consist of ion exchange liquid chromatography (LC), reverse phase LC, carrier ampholyte based separations, e.g. the use of Rotofor, affinity based separations, or gel based separations. Each first-dimension fraction obtained usingone separation mode can be further resolved using one or more of the otherseparation modes to yield either purified protein in solution or liquid fractions with substantially reduced protein complexity. The advantage of a liquid based separation system is that proteins in hundreds of individual fractions can be arrayed directly and used as targets for a variety of probes. Constituent proteins in reactive fractions are identified by mass spectrometry and may be further resolved to determine the nature of the reactive protein(s). We present in this report initial data based on microarray analysis of individual Rotofor fractions obtained from lung adenocarcinoma cell line A549 lysates which have been probed with antibodies against specific proteins.

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Documento generato il 02/04/20 alle ore 19:04:13