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Titolo:
New approaches to proteomic analysis of breast cancer
Autore:
Wulfkuhle, JD; McLean, KC; Paweletz, CP; Sgroi, DC; Trock, BJ; Steeg, PS; Petricoin, EF;
Indirizzi:
US FDA, NCI,CBER, Div Therapeut Prod, Clin Proteom Program, Bethesda, MD 20892 USA US FDA Bethesda MD USA 20892 Clin Proteom Program, Bethesda, MD 20892 USA NCI, Canc Res Ctr, Pathol Lab, Womens Canc Sect, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892 l Lab, Womens Canc Sect, Bethesda, MD 20892 USA NCI, Ctr Canc Res, Pathol Lab, Bethesda, MD 20892 USA NCI Bethesda MD USA20892 tr Canc Res, Pathol Lab, Bethesda, MD 20892 USA Georgetown Univ, Dept Chem, Washington, DC 20057 USA Georgetown Univ Washington DC USA 20057 pt Chem, Washington, DC 20057 USA Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 h Med, Dept Pathol, Boston, MA 02115 USA Massachusetts Gen Hosp, Mol Pathol Unit, Boston, MA 02114 USA Massachusetts Gen Hosp Boston MA USA 02114 hol Unit, Boston, MA 02114 USA Georgetown Univ, Med Ctr, Vincent T Lombardi Canc Res Ctr, Washington, DC 20007 USA Georgetown Univ Washington DC USA 20007 Res Ctr, Washington, DC 20007 USA
Titolo Testata:
PROTEOMICS
fascicolo: 10, volume: 1, anno: 2001,
pagine: 1205 - 1215
SICI:
1615-9853(200110)1:10<1205:NATPAO>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
2-DIMENSIONAL GEL-ELECTROPHORESIS; LASER CAPTURE MICRODISSECTION; MAMMARY EPITHELIAL-CELLS; PROTEIN EXPRESSION; POLYPEPTIDE EXPRESSION; DUCTAL CARCINOMA; RISK; HYPERPLASIA; PROGRESSION; DISEASE;
Keywords:
breast cancer; surface-enhanced laser desorption/ionization two-dimensional polyacrylamide gel electrophoresis; laser capture microdissection pharmacoproteomics; review;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Petricoin, EF US FDA, NCI,CBER, Div Therapeut Prod, Clin Proteom Program, Bldg 29A,Room 2B02,8800 Rockville Pike, Bethesda, MD 20892 USA US FDA Bldg 29A,Room 2B02,8800 Rockville Pike Bethesda MD USA 20892
Citazione:
J.D. Wulfkuhle et al., "New approaches to proteomic analysis of breast cancer", PROTEOMICS, 1(10), 2001, pp. 1205-1215

Abstract

Proteomic based approaches are beginning to be utilized to study the natural history and treatment of breast cancer. A variety of proteomics approaches are under study, and are summarized herein. Two-dimensional gel electrophoresis (2D-PAGE) is still the foundation of most proteomics studies. We present an analysis of 2D-PAGE studies reported to date in breast cancer, including those examining normal/tumor differences and selected populations ofbreast cells. Newer technologies such as laser capture microdissection andhighly sensitive mass spectrometry methods are currently being used together to identify greater numbers of lower abundance proteins that are differentially expressed between defined cell populations. Novel technologies still in developmental phases will enable identification of validated targets in small biopsy specimens, including high density protein arrays, antibody arrays and lysate arrays. Surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) analysis enables the high throughput characterizationof lysates from very few tumor cells and may be best suited for clinical biomarker studies. We present SELDI-TOF data herein to show the accuracy of the method in a small cohort of breast tumors, as well as its potential discriminatory capability. Such technologies are expected to supplement our armamentarium of mRNA-based assays, and provide critical information on protein levels and post-translational modifications.

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Documento generato il 14/07/20 alle ore 06:03:16