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Titolo:
Systemic administration of TerplexDNA system: Pharmacokinetics and gene expression
Autore:
Yu, L; Suh, H; Koh, JJ; Kim, SW;
Indirizzi:
Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Ctr Controlled Chem Delivery, Salt Lake City, UT 84112 USA Univ Utah Salt Lake City UT USA 84112 ivery, Salt Lake City, UT 84112 USA
Titolo Testata:
PHARMACEUTICAL RESEARCH
fascicolo: 9, volume: 18, anno: 2001,
pagine: 1277 - 1283
SICI:
0724-8741(200109)18:9<1277:SAOTSP>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA DELIVERY; IN-VITRO; PROTEOGLYCANS; THERAPY; CELLS;
Keywords:
gene delivery; gene therapy; gene carrier; PK/PD; TerplexDNA system;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, SW Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Ctr Controlled Chem Delivery, Salt Lake City, UT 84112 USA Univ Utah Salt Lake City UT USA 84112 alt Lake City, UT 84112 USA
Citazione:
L. Yu et al., "Systemic administration of TerplexDNA system: Pharmacokinetics and gene expression", PHARM RES, 18(9), 2001, pp. 1277-1283

Abstract

Purpose. The aim of this study is to extend our previous studies to investigate the TerplexDNA synthetic gene carrier system in pharmacokinetics, biodistribution, and gene expression in major organs after systemic administration. Methods. The stability of the TerplexDNA system was analyzed in vitro witha serum incubation assay. The TerplexDNA PK/PD studies were conducted by quantitation of Terplex/radiolabeled DNA [CTP alpha-P-32] complexes after rat-tail vein injection. The effect of the TerplexDNA system on gene expression in mouse major organs was analyzed by measuring luciferase activities after systemic administration. Results. The TerplexDNA gene carrier showed significantly longer retentionin the vascular space than naked plasmid DNA alone. At early time points (1 h postvenous injection), the lung was the major organ of the TerplexDNA distribution, followed by the liver as a major distribution organ at later time points (24 h postinjection). The major organs of transgene expression after intravenous injection were the liver and heart. Conclusion. The TerplexDNA system has the potential for in vivo applications due to its higher bioavailability of plasmid DNA in the tissues, and dueto its organ specific distribution.

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Documento generato il 30/09/20 alle ore 09:21:03