Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Ultrasound-induced hyperthermia increases cellular uptake and cytotoxicityof P-glycoprotein substrates in multi-drug resistant cells
Autore:
Liu, Y; Cho, CW; Yan, XD; Henthorn, TK; Lillehei, KO; Cobb, WN; Ng, KY;
Indirizzi:
Univ Colorado, Hlth Sci Ctr, Sch Pharm, Dept Pharmaceut Sci, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 pt Pharmaceut Sci, Denver, CO 80262 USA Univ Colorado, Hlth Sci Ctr, Sch Med, Dept Neurosurg, Denver, CO 80262 USAUniv Colorado Denver CO USA 80262 d, Dept Neurosurg, Denver, CO 80262 USA Univ Colorado, Hlth Sci Ctr, Sch Pharm, Dept Anesthesiol, Denver, CO 80262USA Univ Colorado Denver CO USA 80262 , Dept Anesthesiol, Denver, CO 80262USA Univ Denver, Res Inst, Denver, CO 80208 USA Univ Denver Denver CO USA 80208 iv Denver, Res Inst, Denver, CO 80208 USA
Titolo Testata:
PHARMACEUTICAL RESEARCH
fascicolo: 9, volume: 18, anno: 2001,
pagine: 1255 - 1261
SICI:
0724-8741(200109)18:9<1255:UHICUA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HAMSTER OVARY CELLS; INTENSITY FOCUSED ULTRASOUND; CYTO-TOXICITY; ADRIAMYCIN; EXPRESSION; GENE; THERMOCHEMOTHERAPY; VINBLASTINE; MECHANISMS; COLCHICINE;
Keywords:
ultrasounds hyperthermia; multi-drug resistance; MDR; P-glycoprotein; P-glycoprotein modulating agents; veraparnil; anticancer drugs;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Ng, KY Univ Colorado, Hlth Sci Ctr, Sch Pharm, Dept Pharmaceut Sci, 4200 E9th Ave, Denver, CO 80262 USA Univ Colorado 4200 E 9th Ave Denver CO USA 80262 nver, CO 80262 USA
Citazione:
Y. Liu et al., "Ultrasound-induced hyperthermia increases cellular uptake and cytotoxicityof P-glycoprotein substrates in multi-drug resistant cells", PHARM RES, 18(9), 2001, pp. 1255-1261

Abstract

Purpose. Localized hyperthermia has been shown previously to augment the cytotoxicity of some lipophilic anticancer drugs. Because many of the substrates for the multi-drug resistance (MDR) transporter P-glycoprotein (P-gp) are lipophilic in nature, studies were conducted to test the hypothesis that hyperthermia induced by ultrasound could also increase cellular uptake and cytotoxicity of P-gp substrates by P-gp-expressing cells. Methods. To test this hypothesis, we studied the effects of hyperthermia and ultrasound on cellular accumulation of putative P-gp substrates, rhodamine 123 (R123) and doxorubicin (DOX), and cytotoxicity of DOX in the parent and MDR variants of two human cancer cell lines. Results. Treatment of cells with hyperthermia or ultrasound (20 min at 41 degreesC) both caused a significant increase over controls (no ultrasound treatment) in R123 and DOX accumulation in the parent and MDR lines of MV522and KB cells. Ultrasound also substantially increased the antiproliferative effects of DOX in both the parent and MDR variants of MV522 and KB cell lines when compared with controls. Our results also indicated that ultrasound exerted a much greater effect on cellular accumulation of R123 and DOX and cytotoxicity enhancement of DOX in the MDR variants than putative P-gp antagonist such as verapamil. Conclusion. The present results point to the potential use of ultrasound-induced hyperthermia as a much safer alternative to P-gp antagonist for reversal of MDR.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 07:06:04