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Titolo:
Cold preservation of rat pancreatic islets just above the freezing point using University of Wisconsin solution
Autore:
Inui, H; Kwon, AH; Yoshida, K; Tsuchiya, H; Inoue, K; Kamiyama, Y;
Indirizzi:
Kansai Med Univ, Dept Surg 1, Moriguchi, Osaka 5708507, Japan Kansai Med Univ Moriguchi Osaka Japan 5708507 guchi, Osaka 5708507, Japan Kyoto Univ, Inst Frontier Med Sci, Dept Organ Reconstruct, Kyoto 6110011, Japan Kyoto Univ Kyoto Japan 6110011 t Organ Reconstruct, Kyoto 6110011, Japan
Titolo Testata:
PANCREAS
fascicolo: 4, volume: 23, anno: 2001,
pagine: 382 - 386
SICI:
0885-3177(200111)23:4<382:CPORPI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIVER PRESERVATION; UW SOLUTION; STORAGE; CRYOPRESERVATION; TRANSPLANTATION; TEMPERATURE;
Keywords:
hyo-on preservation; islet preservation; islet transplantation; UW solution;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Inui, H Kansai Med Univ, Dept Surg 1, 10-15 Fumizono, Moriguchi, Osaka 5708507, Japan Kansai Med Univ 10-15 Fumizono Moriguchi Osaka Japan 5708507 Japan
Citazione:
H. Inui et al., "Cold preservation of rat pancreatic islets just above the freezing point using University of Wisconsin solution", PANCREAS, 23(4), 2001, pp. 382-386

Abstract

Aims: To confirm whether rat islets stored at a temperature just above thefreezing point using University of Wisconsin (UW) solution would remain viable for the short term. Methodology: Rat islets were stored for 24 hours in UW solution, either at4 degreesC or at -0.6 degreesC (just above the specific freezing point of the UW solution). After cold storage, the islets were assessed for in vitroviability by static incubation and for in vivo viability by a transplantation study. One thousand islets preserved under different conditions were injected intraportally into a streptozotocin-induced diabetic rat as an isograft. Four weeks after the transplantation, an intravenous glucose tolerancetest was performed. Results: Islets stored at -0.6 degreesC showed higher insulin secretion rates than those stored at 4 degreesC on a static challenge. The interval from transplantation to the achievement of normoglycemia was also shorter in the -0.6 degreesC group than in the 4 degreesC group. After islet transplantation, the daily nonfasting plasma glucose concentration was higher in the 4 degreesC group than in the -0.6 degreesC group. When compared with the 4 degreesC group, the -0.6 degreesC group showed lower blood glucose values during all investigational periods on an intravenous glucose tolerance test. Conclusion: Islet preservation at -0.6 degreesC using UW solution is more advantageous for short term.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 00:20:34