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Titolo:
Usefulness of supernatant of pancreatic juice for genetic analysis of K-ras in diagnosis of pancreatic carcinoma
Autore:
Ha, A; Watanabe, H; Yamaguchi, Y; Ohtsubo, K; Wang, Y; Motoo, Y; Okai, T; Wakabayahi, T; Sawabu, N;
Indirizzi:
Kanazawa Univ, Inst Canc Res, Dept Internal Med & Med Oncol, Kanazawa, Ishikawa 9218044, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9218044 wa, Ishikawa 9218044, Japan Kanazawa Saiseikai Hosp, Dept Gastroenterol, Kanazawa, Ishikawa, Japan Kanazawa Saiseikai Hosp Kanazawa Ishikawa Japan anazawa, Ishikawa, Japan
Titolo Testata:
PANCREAS
fascicolo: 4, volume: 23, anno: 2001,
pagine: 356 - 363
SICI:
0885-3177(200111)23:4<356:UOSOPJ>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; HYBRIDIZATION PROTECTION ASSAY; POINT MUTATIONS; CYTOLOGIC EXAMINATION; ONCOGENE ACTIVATION; CANCER; CODON-12; ADENOCARCINOMA; DNA; IDENTIFICATION;
Keywords:
K-ras codon 12; pancreatic carcinoma; MASA; pancreatic juice; supernatant;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Sawabu, N Kanazawa Univ, Inst Canc Res, Dept Internal Med & Med Oncol, 4-86 Yoncizumi, Kanazawa, Ishikawa 9218044, Japan Kanazawa Univ 4-86 YoncizumiKanazawa Ishikawa Japan 9218044 pan
Citazione:
A. Ha et al., "Usefulness of supernatant of pancreatic juice for genetic analysis of K-ras in diagnosis of pancreatic carcinoma", PANCREAS, 23(4), 2001, pp. 356-363

Abstract

Aims: To ascertain whether analysis of K-ras mutations at codon 12 (KRM) in the supernatant of pure pancreatic juice (PPJ) is more useful for the diagnosis of pancreatic carcinoma (PCa) than that in sediment, the authors analyzed KRM in DNA extract from not only the sediment but also the supernatant of PPJ and compared the results. Methodology: PPJ was collected endoscopically from 19 patients with PCa and 25 patients with chronic pancreatitis (CP). DNA was extracted from the supernatant and the sediment of PPJ. Mutant allele-specific amplification (MASA) was performed for KRM analysis with the DNA extracts from these samples. Results: The incidence of KRM in the supernatant of PPJ was 89% (17 of 19)in patients with PCa and 28% (7 of 25) in patients with CP, whereas that in the sediment was 79% (15 of 19) in patients with PCa and 20% (5 of 25) inpatients with CP. Although there was no significant difference in KRM incidence between supernatant and sediment, the positive rate of KRM was higherin the former. Additionally, with regard to the PCa cases, KRM were found in the supernatant alone in four cases and in the sediment alone in two cases. Consequently, by a combination assay, ail of the patients with PCa showed KRM in either the supernatant or sediment of PPJ. Although there was no relation between the incidence of KRM in PPJ and the location and size of tumor. and clinical stage of carcinoma in the patients with PCa. two patients with clinical stage I disease showed KRM in the supernatant. Conclusion: These results suggest that the positive rate of KRM in the supernatant is not lower than that in the sediment, and simultaneous analysis of KRM in the supernatant and sediment of PPJ enhances the genetic diagnosis of PCa.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 09:23:48