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Titolo:
Synergistic effects of clinically achievable concentrations of 12-O-tetradecanoylphorbol-13-acetate in combination with all-trans retinoic acid, 1 alpha,25-dihydroxyvitamin D-3, and sodium butyrate on differentiation in HL-60 cells
Autore:
Zheng, X; Chang, RL; Cui, XX; Kelly, KA; Shih, WCJ; Lin, Y; Strair, R; Suh, J; Han, ZT; Rabson, A; Conney, AH;
Indirizzi:
Rutgers State Univ, Coll Pharm, Dept Biol Chem, Canc Res Lab, Piscataway, NJ 08854 USA Rutgers State Univ Piscataway NJ USA 08854 Lab, Piscataway, NJ 08854 USA Univ Med & Dent New Jersey, Sch Publ Hlth, Div Biometr, New Brunswick, NJ 08903 USA Univ Med & Dent New Jersey New Brunswick NJ USA 08903 swick, NJ 08903 USA Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Canc Inst New Jersey, New Brunswick, NJ 08903 USA Univ Med & Dent New Jersey New Brunswick NJ USA 08903 swick, NJ 08903 USA Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Ctr Adv Biotechnol & Med, Piscataway, NJ 08854 USA Univ Med & Dent New Jersey Piscataway NJUSA 08854 scataway, NJ 08854 USA Henan Tumor Res Inst, Zhengzhou 450000, Henan, Peoples R China Henan TumorRes Inst Zhengzhou Henan Peoples R China 450000 oples R China
Titolo Testata:
ONCOLOGY RESEARCH
fascicolo: 9-10, volume: 12, anno: 2001,
pagine: 419 - 427
SICI:
0965-0407(2001)12:9-10<419:SEOCAC>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE PROMYELOCYTIC LEUKEMIA; PROTEIN-KINASE-C; BREAST-CANCER CELLS; PHORBOL ESTER; MACROPHAGE DIFFERENTIATION; NB4 CELLS; IN-VITRO; INDUCTION; TRIBUTYRIN; ANALOGS;
Keywords:
TPA; PMA; leukemia; growth; differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Conney, AH Rutgers State Univ, Coll Pharm, Dept Biol Chem, Canc Res Lab, Piscataway, NJ 08854 USA Rutgers State Univ Piscataway NJ USA 08854 taway, NJ 08854 USA
Citazione:
X. Zheng et al., "Synergistic effects of clinically achievable concentrations of 12-O-tetradecanoylphorbol-13-acetate in combination with all-trans retinoic acid, 1 alpha,25-dihydroxyvitamin D-3, and sodium butyrate on differentiation in HL-60 cells", ONCOL RES, 12(9-10), 2001, pp. 419-427

Abstract

Our recent studies demonstrated that 12-O-tetradecanoylphorbol-13-acetate (TPA) has pharmacological activity for the treatment of acute myelocytic leukemia patients. In the present study, we investigated the potential synergistic effect of all-trans retinoic acid (RA), 1 alpha ,25-dihydroxyvitamin D-3 (VD3), and sodium butyrate (NaB) on TPA-induced differentiation in HL-60 human promyelocytic leukemia cells. The cells were treated once with these agents for 48 h or treated every 24 h for 96 h. Treatment of HL-60 cells once with TPA, RA, VD3, or NaB for 48 h resulted in concentration-dependentgrowth inhibition and cell differentiation. At clinically achievable concentrations, TPA (0.16 nM) increased the number of adherent cells and RA (0.1-1 muM) increased the number of nitroblue tetrazolium (NDT)-positive cells. The combinations of TPA (0.16 nM) with RA (0.1-1 muM), VD3 (1 nM), or NaB (100 muM) for 48 h synergistically increased differentiation as measured bythe formation of adherent cells (P less than or equal to 0.01). Moreover, cells treated with various combinations of low concentrations of TPA, RA, VD3, and NaB every 24 h for 96 h resulted in a further decrease in cell growth and an increase in differentiation. At clinically achievable concentrations, the strongest stimulation of differentiation was achieved in cells treated with a "cocktail" that combined TPA, RA, VD3, and NaB. The synergisticeffect of combinations of TPA with RA or NaB at clinically effective concentrations on HL-60 cell differentiation suggests that the combination of these agents may improve the therapeutic efficacy of TPA for the treatment ofacute promyelocytic leukemia (APL) patients. A differentiation "cocktail" that combines TPA, RA, VD3, and NaB may provide an even more effective strategy for improving the therapeutic efficacy of TPA and RA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 00:38:58