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Titolo:
A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences
Autore:
Nagano, Y; Furuhashi, H; Inaba, T; Sasaki, Y;
Indirizzi:
Nagoya Univ, Grad Sch Agr Sci, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 Sci, Nagoya, Aichi 4648601, Japan
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 20, volume: 29, anno: 2001,
pagine: 4097 - 4105
SICI:
0305-1048(20011015)29:20<4097:ANCOPZ>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOWN-REGULATED EXPRESSION; TRANSCRIPTION FACTORS; PEA; DOMAIN; EUKARYOTES; PROMOTER; FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Nagano, Y Nagoya Univ, Grad Sch Agr Sci, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 ya, Aichi 4648601, Japan
Citazione:
Y. Nagano et al., "A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences", NUCL ACID R, 29(20), 2001, pp. 4097-4105

Abstract

Complementary DNA encoding. a DNA-binding protein, designated PLATZ1 (plant AT-rich sequence-and zinc-binding protein 1), was isolated from peas. Theamino acid sequence of the protein is similar to those of other uncharacterized proteins predicted from the genome sequences of higher plants. However, no paralogous sequences have been found outside the plant kingdom. Multiple alignments among these paralogous proteins show that several cysteine and histidine residues are invariant, suggesting that these proteins are a novel class of zinc-dependent DNA-binding proteins with two distantly located regions, C-x(2)-H-x(11)-C-x(2-)C-x((4-5))-C- x(2)-C-x((3-7))-H-x(2)-H andC-x(2)-C-x((10-11))-C-x(3)-C. In an electrophoretic mobility shift assay, the zinc chelator 1,10-o-phenanthroline inhibited DNA binding, and two distant zinc-binding regions were required for DNA binding. A protein blot with(ZnCl2)-Zn-65 showed that both regions are required for zinc-binding activity. The PLATZ1 protein non-specifically binds to A/T-rich sequences, including the upstream region of the pea GTPase pra2 and plastocyanin petE genes. Expression of the PLATZ1 repressed those of the reporter constructs containing the coding sequence of luciferase gene driven by the cauliflower mosaic virus (CaMV) 35S90 promoter fused to the tandem repeat of the A/T-rich sequences. These results indicate that PLATZ1 is a novel class of plant-specific zinc-dependent DNA-binding protein responsible for A/T-rich sequence-mediated transcriptional repression.

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Documento generato il 03/04/20 alle ore 08:01:53