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Titolo:
Regulation of the ecto-nucleotidase pathway in rat hippocampal nerve terminals
Autore:
Cunha, RA;
Indirizzi:
Univ Lisbon, Fac Sci, Dept Chem & Biochem, P-1699 Lisbon, Portugal Univ Lisbon Lisbon Portugal P-1699 em & Biochem, P-1699 Lisbon, Portugal Univ Lisbon, Fac Med, Neurosci Lab, P-1699 Lisbon, Portugal Univ Lisbon Lisbon Portugal P-1699 Neurosci Lab, P-1699 Lisbon, Portugal
Titolo Testata:
NEUROCHEMICAL RESEARCH
fascicolo: 8-9, volume: 26, anno: 2001,
pagine: 979 - 991
SICI:
0364-3190(200109)26:8-9<979:ROTEPI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRACELLULAR ADENINE-NUCLEOTIDES; ADENOSINE RECEPTOR ACTIVATION; INNERVATED SKELETAL-MUSCLE; TORPEDO ELECTRIC ORGAN; ATP-DIPHOSPHOHYDROLASE; ENDOGENOUS ADENOSINE; ENDOTHELIAL-CELLS; CEREBRAL-CORTEX; NEUROMUSCULAR-JUNCTION; ACETYLCHOLINE-RELEASE;
Keywords:
ecto-nucleotidases; ecto-5 '-nucleotidase; ATP; adenosine; hippocampus nerve terminals;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Cunha, RA Univ Coimbra, Fac Med, Ctr Neurosci Coimbra, P-3004504 Coimbra, Portugal Univ Coimbra Coimbra Portugal P-3004504 4504 Coimbra, Portugal
Citazione:
R.A. Cunha, "Regulation of the ecto-nucleotidase pathway in rat hippocampal nerve terminals", NEUROCHEM R, 26(8-9), 2001, pp. 979-991

Abstract

Ecto-nucleotidases play a pivotal role in terminating the signalling via ATP and in producing adenosine, a neuromodulator in the nervous system. We have now investigated the pattern of adenosine formation with different concentrations of extracellular ATP in rat hippocampal nerve terminals. It was found that adenosine formation is delayed with increasing concentrations ofATP. Also, the rate of adenosine formation increased sharply when the extracellular concentrations of ATP + ADP decrease below 5 muM, indicating thatATP/ADP feed-forwardly inhibit ecto-5'-nucleotidase allowing a burst-like formation of adenosine possibly designed to activate facilitatory A(2A) receptors. Initial rate measurements of ecto-5'-nucleotidase in hippocampal nerve terminals, using IMP as substrate, showed that ATP and ADP are competitive inhibitors (apparent K-i of 14 and 4 muM). In contrast, in hippocampal immunopurified cholinergic nerve terminals, a burst-like formation of adenosine is not apparent, suggesting that channelling processes may overcome the feed-forward inhibition of ecto-5'-nucleotidase, thus favouring A(1) receptor activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:32:53