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Titolo:
Protein kinase C is involved in M-1-muscarinic receptor-mediated facilitation of L-type Ca2+ channels in neurons of the major pelvic ganglion of the adult male rat
Autore:
Sculptoreanu, A; Yoshimura, N; de Groat, WC; Somogyi, GT;
Indirizzi:
Univ Pittsburgh, Sch Med, Dept Pharmacol, Pittsburgh, PA 15261 USA Univ Pittsburgh Pittsburgh PA USA 15261 armacol, Pittsburgh, PA 15261 USA
Titolo Testata:
NEUROCHEMICAL RESEARCH
fascicolo: 8-9, volume: 26, anno: 2001,
pagine: 933 - 942
SICI:
0364-3190(200109)26:8-9<933:PKCIII>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRESYNAPTIC MUSCARINIC RECEPTORS; BETA-GAMMA-SUBUNITS; CALCIUM CHANNELS; URINARY-BLADDER; N-TYPE; SYMPATHETIC NEURONS; GUINEA-PIG; NEUROTRANSMITTER RELEASE; ACETYLCHOLINE-RELEASE; NORADRENALINE RELEASE;
Keywords:
cholinergic innervation; L-typeCa(2+) channels; muscarinic signaling; major pelvic ganglion; urinary bladder;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Sculptoreanu, A Univ Pittsburgh, Sch Med, Dept Pharmacol, E1304 Biomed SciTower, Pittsburgh, PA 15261 USA Univ Pittsburgh E1304 Biomed Sci Tower Pittsburgh PA USA 15261
Citazione:
A. Sculptoreanu et al., "Protein kinase C is involved in M-1-muscarinic receptor-mediated facilitation of L-type Ca2+ channels in neurons of the major pelvic ganglion of the adult male rat", NEUROCHEM R, 26(8-9), 2001, pp. 933-942

Abstract

We used patch clamp recording techniques to determine if muscarinic signaling mechanisms are present in dissociated autonomic neurons obtained from the major pelvic ganglion, which provides the cholinergic innervation of theurinary bladder and other pelvic organs. The M-1 specific agonist, McN-A-343 (2-30 muM) enhanced Ca2+ currents in approximately 37% of neurons (by 50-80%). This enhancement was reduced by atropine (5-10 muM) or a PKC inhibitor (bisindolylmaleimide, 50-200 nM). In responsive neurons Ca2+ currents were also enhanced by the phorbol ester, phorbol-12, 13-dibutyrate (50-300 nM) and the dihydropyridine agonist Bay K 8644 (5 muM) and had kinetics of activation and inactivation as expected for L-type Ca2+ channels, We concludethat in a subpopulation of MPG neurons, M1-mediated activation of PKC phosphorylates and enhances L-type Ca2+ channel activities. This muscarinic facilitatory mechanism in MPG neurons may be the same as the M1-mediated facilitation of transmitter release reported previously at the nerve terminals in the urinary bladder.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 04:06:31