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Titolo:
A murine dopamine neuron-specific cDNA library and microarray: Increased COXI expression during methamphetamine neurotoxicity
Autore:
Barrett, T; Xie, T; Piao, Y; Dillon-Carter, O; Kargul, GJ; Lim, MK; Chrest, FJ; Wersto, R; Rowley, DL; Juhaszova, M; Zhou, L; Vawter, MP; Becker, KG; Cheadle, C; Wood, WH; McCann, UD; Freed, WJ; Ko, MS; Ricaurte, GA; Donovan, DM;
Indirizzi:
NIA, Intramural Res Program, Res Resources Branch, NIH, Baltimore, MD 21224 USA NIA Baltimore MD USA 21224 Resources Branch, NIH, Baltimore, MD 21224 USA NIA, Genet Lab, NIH, Baltimore, MD 21224 USA NIA Baltimore MD USA 21224NIA, Genet Lab, NIH, Baltimore, MD 21224 USA NIDA, Cellular Neurobiol Branch, NIH, Baltimore, MD 21224 USA NIDA Baltimore MD USA 21224 eurobiol Branch, NIH, Baltimore, MD 21224 USA Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 Neurol, Baltimore, MD 21205 USA Johns Hopkins Univ, Sch Med, Dept Psychiat & Behav Sci, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 av Sci, Baltimore, MD 21205 USA
Titolo Testata:
NEUROBIOLOGY OF DISEASE
fascicolo: 5, volume: 8, anno: 2001,
pagine: 822 - 833
SICI:
0969-9961(200110)8:5<822:AMDNCL>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSITRON-EMISSION-TOMOGRAPHY; CENTRAL-NERVOUS-SYSTEM; HEAT-SHOCK-PROTEIN; STRIATAL DOPAMINE; INDUCED HYPERTHERMIA; RAT-BRAIN; OXIDATIVE STRESS; C-11 WIN-35,428; MESSENGER-RNAS; MICE;
Keywords:
dopamine; cDNA library; cDNA microarray; methamphetamine; development; gene expression profile;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Barrett, T NIA, Intramural Res Program, Res Resources Branch, NIH, 5600 Nathan Shock Dr, Baltimore, MD 21224 USA NIA 5600 Nathan Shock Dr Baltimore MD USA 21224 e, MD 21224 USA
Citazione:
T. Barrett et al., "A murine dopamine neuron-specific cDNA library and microarray: Increased COXI expression during methamphetamine neurotoxicity", NEUROBIOL D, 8(5), 2001, pp. 822-833

Abstract

Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter-lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greaterthan twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantial nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 13:15:52