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Titolo:
Molecular studies on intraspecific diversity and phylogenetic position of Coniothyrium minitans
Autore:
Muthumeenakshi, S; Goldstein, AL; Stewart, A; Whipps, JM;
Indirizzi:
Hort Res Int, Plant Pathol & Microbiol Dept, Warwick CV35 9EF, England Hort Res Int Warwick England CV35 9EF ol Dept, Warwick CV35 9EF, England Lincoln Univ, Soil Plant & Ecol Sci Div, Canterbury, New Zealand Lincoln Univ Canterbury New Zealand ol Sci Div, Canterbury, New Zealand
Titolo Testata:
MYCOLOGICAL RESEARCH
, volume: 105, anno: 2001,
parte:, 9
pagine: 1065 - 1074
SICI:
0953-7562(200109)105:<1065:MSOIDA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL DNA-SEQUENCES; MULTILOCUS ENZYME ELECTROPHORESIS; SCLEROTINIA-SCLEROTIORUM; TRICHODERMA-HARZIANUM; BIOLOGICAL-CONTROL; MUSHROOM COMPOST; CANDIDA-ALBICANS; BRITISH-ISLES; FUNGI; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Goldstein, AL Duke Univ, Med Ctr, Dept Microbiol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 crobiol, Durham, NC 27710 USA
Citazione:
S. Muthumeenakshi et al., "Molecular studies on intraspecific diversity and phylogenetic position of Coniothyrium minitans", MYCOL RES, 105, 2001, pp. 1065-1074

Abstract

Simple sequence repeat (SSR)-PCR amplification using a microsatellite primer (GACA)(4) and ribosomal RNA gene sequencing were used to examine the intraspecific diversity in the mycoparasite Coniothyrium minitans based on 48 strains, representing eight colony types, from 17 countries world-wide, Coniothyrium cerealis, C. fuckelii and C. sporulosum were used for interspecific comparison. The SSR-FCR technique revealed a relatively low level of polymorphism within C. minitans but did allow some differentiation between strains. While there was no relationship between SSR-PCR profiles and colony type, there was some limited correlation between these profiles and country of origin. Sequences of the ITS I and ITS 2 regions and the 5.8S gene of rRNA genes were identical in all twenty-four strains of C. minitans examined irrespective of colony type and origin. These results indicate that C. minitans is genetically not very variable despite phenotypic differences. ITS and 5.8S rRNA gene sequence analyses showed that C. minitans had similarities of 94 % with C. fuckelii and C. sporulosum (which were identical to each other) and only 64 % with C. cerealis. Database searches failed to show anysimilarity with the ITS 1 sequence for C. minitans although the 5.8S rRNA gene and ITS 2 sequences revealed an 87% similarity with Aporospora terricola. The ITS sequence including the 5.8S rRNA gene sequence of Coniothyrium cerealis showed 91% similarity to Phaeosphaeria microscopica. Phylogenetic analyses using database information suggest that C. minitans, C. sporulosum, C. fuckelii and A. terricola duster in one clade, grouping with Helminthosporium species and 'Leptosphaeria' bicolor. Coniothyrium cerealis grouped with Ampelomyces quisqualis and formed a major cluster with members of the Phaeosphaeriacae and Phaeosphaeria microscopica.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:44:41