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Titolo:
Physical mapping across an avirulence locus of Phytophthora infestans using a highly representative, large-insert bacterial artificial chromosome library
Autore:
Whisson, SC; van derLee, T; Bryan, GJ; Waugh, R; Govers, F; Birch, PRJ;
Indirizzi:
Scottish Crop Res Inst, Dundee DD2 5DA, Scotland Scottish Crop Res Inst Dundee Scotland DD2 5DA Dundee DD2 5DA, Scotland Univ Wageningen & Res Ctr, Grad Sch Expt Plant Sci, Phytopathol Lab, NL-6709 PD Wageningen, Netherlands Univ Wageningen & Res Ctr Wageningen Netherlands NL-6709 PD Netherlands
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 2, volume: 266, anno: 2001,
pagine: 289 - 295
SICI:
1617-4615(200110)266:2<289:PMAAAL>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
F-SP GLYCINEA; LINKAGE MAP; GENE; CONSTRUCTION; EXPRESSION; DNA; TRANSFORMATION; ORGANIZATION; POTATO; AFLP;
Keywords:
plant pathogen; genomics; oomycete; tomato;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Whisson, SC Scottish Crop Res Inst, Dundee DD2 5DA, Scotland Scottish CropRes Inst Dundee Scotland DD2 5DA 5DA, Scotland
Citazione:
S.C. Whisson et al., "Physical mapping across an avirulence locus of Phytophthora infestans using a highly representative, large-insert bacterial artificial chromosome library", MOL GENET G, 266(2), 2001, pp. 289-295

Abstract

The oomycete plant pathogen Phytophthora infestans is the causal agent of late blight, one of the most devastating diseases of potato worldwide. As part of efforts to clone avirulence (Avr) genes and pathogenicity factors from P. infestans, we have constructed a bacterial artificial chromosome (BAC) library from an isolate containing six Avr genes. The BAC library comprises clones with an average insert size of 98 kb and represents an estimated 10 genome equivalents. A three-dimensional pooling strategy was developed to screen the BAC library for amplified fragment length polymorphism (AFLP) markers, as this type of marker has been extensively used in construction of a P. infestans genetic map. Multiple positive clones were identified for each AFLP marker tested. The pools were used to construct a contig of 11 BAC clones in a region of the P. infestans genome containing a cluster of three avirulence genes. The BAC contig is predicted to encompass the Avr11 locus but mapping of the BAC ends will be required to determine if the Avr3 and Avr10 loci are also present in the BAC contig. These results are an important step towards the positional cloning of avirulence genes from P. infestans, and the BAC library represents a valuable resource for large-scale studies of oomycete genome organisation and gene content.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:41:01