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Titolo:
Identification of an 85-kb DNA fragment containing pms1, a locus for photoperiod-sensitive genic male sterility in rice
Autore:
Liu, N; Shan, Y; Wang, FP; Xu, CG; Peng, KM; Li, XH; Zhang, QF;
Indirizzi:
Huazhong Agr Univ, Natl Key Lab Genet Crop Improvement, Wuhan 430070, Peoples R China Huazhong Agr Univ Wuhan Peoples R China 430070 n 430070, Peoples R China
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 2, volume: 266, anno: 2001,
pagine: 271 - 275
SICI:
1617-4615(200110)266:2<271:IOA8DF>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR MAP; GENES;
Keywords:
pms1; fine-scale mapping; chromosomal walking; BAC contig; molecular marker;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Zhang, QF Huazhong Agr Univ, Natl Key Lab Genet Crop Improvement, Wuhan 430070, Peoples R China Huazhong Agr Univ Wuhan Peoples R China 430070 Peoples R China
Citazione:
N. Liu et al., "Identification of an 85-kb DNA fragment containing pms1, a locus for photoperiod-sensitive genic male sterility in rice", MOL GENET G, 266(2), 2001, pp. 271-275

Abstract

Photoperiod-sensitive genic male-sterile rice has a number of desirable characteristics for hybrid rice production. Previous studies identified pins], located on chromosome 7, as a major locus for photoperiod-sensitive genicmale sterility. The objective of this study was to localize the pins] locus to a specific DNA fragment by genetic and physical mapping. Using 240 highly sterile individuals and a random sample of 599 individuals from an Fz population of over 5000 individuals from a cross between Minghui 63 and 32001S, we localized the pins] locus by molecular marker analysis to a genetic interval of about 4 cM, 0.25 cM from RG477 on one side and 3.8 cM from R1807 on the other side. A contig map composed of seven BAC clones spanning approximate 500 kb in length was constructed for the pms1 region by screening a BAC library of Minghui 63 DNA using RFLP markers and chromosomal walking. Analysis of recombination events in the pms1 region among the highly sterile individuals reduced the length of the contig map to three BAC clones. Sequencing of one BAC clone, 21O9, identified two SSR markers located 85 kb apart in the clone that flanked the pms1 locus on both sides, as indicated by the distribution of recombination events. We thus concluded that the pms1locus was located on the fragment bounded by the two SSR markers.

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Documento generato il 02/04/20 alle ore 12:29:57