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Titolo:
A defined level of protein disulfide isomerase expression is required for optimal secretion of thaumatin by Aspergillus awamori
Autore:
Moralejo, FJ; Watson, AJ; Jeenes, DJ; Archer, DB; Martin, JF;
Indirizzi:
Inst Biotechnol INBIOTEC, Leon 24006, Spain Inst Biotechnol INBIOTEC Leon Spain 24006 ol INBIOTEC, Leon 24006, Spain AFRC, Inst Food Res, Norwich NR4 7UA, Norfolk, England AFRC Norwich Norfolk England NR4 7UA s, Norwich NR4 7UA, Norfolk, England Univ Nottingham, Sch Life & Environm Sci, Nottingham NG7 2RD, England UnivNottingham Nottingham England NG7 2RD , Nottingham NG7 2RD, England
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 2, volume: 266, anno: 2001,
pagine: 246 - 253
SICI:
1617-4615(200110)266:2<246:ADLOPD>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; ENDOPLASMIC-RETICULUM; NIGER; GENE; STRAIN; YEAST;
Keywords:
protein secretion; protein disulfide isomerase; protein folding; thaumatin production; Aspergillus awamori;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Martin, JF Inst Biotechnol INBIOTEC, Sci Pk Leon,Avda Real 1, Leon 24006, Spain Inst Biotechnol INBIOTEC Sci Pk Leon,Avda Real 1 Leon Spain 24006
Citazione:
F.J. Moralejo et al., "A defined level of protein disulfide isomerase expression is required for optimal secretion of thaumatin by Aspergillus awamori", MOL GENET G, 266(2), 2001, pp. 246-253

Abstract

Thaumatin, a 22-kDa protein containing eight disulfide bonds, is secreted by the filamentous fungus Aspergillus awamori at levels which are dependentupon the extent of overexpression of protein disulfide isomerase (PDIA). Additional copies of the PDIA-encoding gene pdiA were introduced into a strain of A. awamori that expresses a cassette encoding thaumatin. Transformants with different levels of pdiA mRNA and measured PDIA levels were chosen for examination of the impact that PDIA levels had on thaumatin secretion. The secretion of two native proteins, alpha -amylase and acid phosphatase, was also examined in relation to varying levels of PDIA. Over a range of PDIA levels of 1-8, relative to the native level in strains with just one copyof the pdiA gene, the fraction of alpha -amylase and acid phosphatase in the total secreted protein was unaffected. In contrast, a peak level of thaumatin, about 5-fold higher than in the strain with one copy of pdiA, was found in strains with a relative PDIA level of between two and four. Improvedthaumatin production was confirmed in 5-1 fermenters using a strain of A. awamori with six pdiA gene copies, containing 3.2-fold higher levels of PDIA than wild-type strains.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 21:28:53