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Titolo:
Arrest of cell division and nucleoid partition by genetic alterations in the sliding clamp of the replicase and in DnaA
Autore:
Kawakami, H; Iwura, T; Takata, M; Sekimizu, K; Hiraga, S; Katayama, T;
Indirizzi:
Kyushu Univ, Grad Sch Pharmaceut Sci, Dept Mol Microbiol, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ol, Higashi Ku, Fukuoka 8128582, Japan Univ Tokyo, Grad Sch Pharmaceut Sci, Lab Dev Biochem, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Biochem, Bunkyo Ku, Tokyo 1130033, Japan Kumamoto Univ, Inst Mol Embryol & Genet, Dept Mol Cell Biol, Kumamoto 8620976, Japan Kumamoto Univ Kumamoto Japan 8620976 Cell Biol, Kumamoto 8620976, Japan
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 2, volume: 266, anno: 2001,
pagine: 167 - 179
SICI:
1617-4615(200110)266:2<167:AOCDAN>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-III HOLOENZYME; ESCHERICHIA-COLI K-12; BETA-SUBUNIT; CHROMOSOMAL REPLICATION; INITIATOR PROTEIN; AUTO-REGULATION; SMALLER FORM; FTSZ RING; IN-VITRO; PCNA;
Keywords:
cell cycle; DnaA; FtsZ; nucleoid; sliding clamp;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Katayama, T Kyushu Univ, Grad Sch Pharmaceut Sci, Dept Mol Microbiol, Higashi Ku, 3-1-1 Maidashi, Fukuoka 8128582, Japan Kyushu Univ 3-1-1 Maidashi Fukuoka Japan 8128582 28582, Japan
Citazione:
H. Kawakami et al., "Arrest of cell division and nucleoid partition by genetic alterations in the sliding clamp of the replicase and in DnaA", MOL GENET G, 266(2), 2001, pp. 167-179

Abstract

In Escherichia coli, an interaction between the replication initiator DnaAand the sliding clamp protein, the beta subunit (DnaN) of DNA polymerase III, is required to regulate the chromosomal replication cycle. We report here that colony formation by, and cell division of, the temperature (42 degreesC)-sensitive dnaN59 mutant are inhibited at 34-35 degreesC when DnaA is moderately (4-to 8-fold) overexpressed, although chromosomal replication and the beta subunit-dependent regulation of DnaA activity are not significantly inhibited. Immunoblotting analysis revealed that the beta subunit is abundant (present at a level of about 5000 dimers per cell) at 34 degreesC, and its concentration per unit cell volume was practically unaffected in thednaN59 mutant by the overexpression of DnaA. The dnaN mutant cells that overexpress DnaA become filamentous at 34 degreesC via an sfiA-independent pathway, different from that activated by the SOS response. This filamentation is accompanied by inhibition of nucleoid partition and FtsZ ring formation. In the dnaN59 mutant, oversupply of DnaA may disturb the coordinated action of cell cycle-regulating molecules, thus leading to the inhibition of these events.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 04:26:15