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Titolo:
Involvement of VIP on BDNF-induced somatostatin gene expression in cultured fetal rat cerebral cortical cells
Autore:
Villuendas, G; Sanchez-Franco, F; Palacios, N; Fernandez, M; Cacicedo, L;
Indirizzi:
Hosp Ramon & Cajal, Serv Endocrinol, Madrid 28034, Spain Hosp Ramon & Cajal Madrid Spain 28034 rv Endocrinol, Madrid 28034, Spain Hosp Carlos III, Madrid 28029, Spain Hosp Carlos III Madrid Spain 28029Hosp Carlos III, Madrid 28029, Spain
Titolo Testata:
MOLECULAR BRAIN RESEARCH
fascicolo: 1-2, volume: 94, anno: 2001,
pagine: 59 - 66
SICI:
0169-328X(20011019)94:1-2<59:IOVOBS>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASOACTIVE-INTESTINAL-PEPTIDE; CILIARY NEUROTROPHIC FACTOR; NEUROPEPTIDE EXPRESSION; SYMPATHETIC NEURONS; NERVOUS-SYSTEM; MESSENGER-RNA; HIPPOCAMPAL INTERNEURONS; INDUCED DEPOLARIZATION; CEREBROCORTICAL CELLS; SENSORY NEURONS;
Keywords:
somatostatin; BDNF; VIP; neuronal activity; neuropeptide; neurotrophin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Cacicedo, L Hosp Ramon & Cajal, Serv Endocrinol, Carretera Colmenar Km 9, Madrid 28034, Spain Hosp Ramon & Cajal Carretera Colmenar Km 9 Madrid Spain 28034
Citazione:
G. Villuendas et al., "Involvement of VIP on BDNF-induced somatostatin gene expression in cultured fetal rat cerebral cortical cells", MOL BRAIN R, 94(1-2), 2001, pp. 59-66

Abstract

Previous studies have shown that BDNF promotes expression of SS. In earlier studies we demonstrated the stimulatory effect of locally produced VIP upon SS secretion. These facts led us to explore the peptidergic action of BDNF on VIP, and to determine if BDNF-induced SS might be mediated by the induction of VIP. Cultured fetal rat cerebralcortical cells were incubated with BDNF (50 ng/ml) and/or VIP (10(-11) M) for 2 and 5 days. In other experiments I-Gs from BDNF or VIP antisera were also added. BDNF increased VIP andSS gene expression and peptide production. After 2 days of incubation withboth BDNF and VIP the induction of SS mRNA was similar to that obtained with BDNF alone. However when the treatment was extended to 5 days the increase in SS mRNA was higher than that obtained with BDNF alone. This finding suggests the possibility that both factors acted synergistically. To define the potential role of VIP in the response of SS gene expression to BDNF, endogenous VIP was blocked with IgGs from VIP antiserum. Under these experimental conditions BDNF-induced SS decreased. Our study provides the first evidence that BDNF up-regulates VIP gene expression and concentration of the peptide. The involvement of VIP on BDNF-induced SS gene expression is also demonstrated. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:51:51