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Titolo:
Ex vivo MR microimaging of neuronal damage after kainate-induced status epilepticus in rat: Correlation with quantitative histology
Autore:
Pirttila, TRM; Pitkanen, A; Tuunanen, J; Kauppinen, RA;
Indirizzi:
Univ Kuopio, AI Virtanen Inst Mol Sci, Epilepsy Res Grp, FIN-70211 Kuopio,Finland Univ Kuopio Kuopio Finland FIN-70211 y Res Grp, FIN-70211 Kuopio,Finland Kuopio Univ Hosp, Dept Neurol, SF-70210 Kuopio, Finland Kuopio Univ Hosp Kuopio Finland SF-70210 eurol, SF-70210 Kuopio, Finland
Titolo Testata:
MAGNETIC RESONANCE IN MEDICINE
fascicolo: 5, volume: 46, anno: 2001,
pagine: 946 - 954
SICI:
0740-3194(200111)46:5<946:EVMMON>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAGNETIC-RESONANCE MICROSCOPY; APPARENT DIFFUSION-COEFFICIENT; KAINIC ACID; AMYGDALOID COMPLEX; BRAIN; SEIZURES; ORGANIZATION; HIPPOCAMPUS; TIME;
Keywords:
amygdala; epilepsy; hippocampus; kainic acid; magnetic resonance microscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Kauppinen, RA Univ Kuopio, AI Virtanen Inst Mol Sci, Natl Bio NMR Facil, POB 1627, FIN-70211 Kuopio, Finland Univ Kuopio POB 1627 Kuopio Finland FIN-70211 opio, Finland
Citazione:
T.R.M. Pirttila et al., "Ex vivo MR microimaging of neuronal damage after kainate-induced status epilepticus in rat: Correlation with quantitative histology", MAGN RES M, 46(5), 2001, pp. 946-954

Abstract

The present study was designed to investigate whether T-2-weighted signal changes obtained by microimaging of paraformaldehyde-fixed brain correlate with the histologically quantified damage in a model of status epilepticus (SE) induced by kainic acid in the rat. Animals were killed at several timepoints up to 8 weeks after a single intraperitoneal kainate (KA) injection(9 mg/kg). Perfusion-fixed brains were embedded in gelatin for MR microlmaging at 9.4T. After the MRI analysis, the gelatin was removed and the brains were cryoprotected and processed for quantitative histology. Severity of neuronal damage and gliosis were assessed from thionin-stained serial sections. Correlative analysis of microimaging and histology data was done in the hippocampus, amygdala, parietal rhinal cortex (PaRH), piriform cortex (Pir), and entorhinal cortex. The relative signal intensities in T-2-weighted images correlate with the severity of neuronal damage in the matched histological sections (correlation coefficients of 0.752-0.826). Our data show that MR microimaging ex vivo detects the degree of neuronal damage and its anatomical distribution after KA-induced SE, thus providing a useful tool fordetecting the dynamics of progressive neuronal damage after prolonged seizures. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:25:42