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Titolo:
Effect of the geometry of microfabricated flow reactors on chemiluminescent detection of epinephrine with lucigenin
Autore:
Kamidate, T; Kaide, T; Tani, H; Makino, E; Shibata, T;
Indirizzi:
Hokkaido Univ, Grad Sch Engn, Div Mol Chem, Kita Ku, Sapporo, Hokkaido 0608628, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608628 oro, Hokkaido 0608628, Japan
Titolo Testata:
LUMINESCENCE
fascicolo: 5, volume: 16, anno: 2001,
pagine: 337 - 342
SICI:
1522-7235(200109/10)16:5<337:EOTGOM>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAPILLARY-ELECTROPHORESIS; CATECHOLAMINES; MICROCHIP; ASSAY;
Keywords:
chemiluminescence; lucigenin; epinephrine; microanalysis system; FIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Kamidate, T Hokkaido Univ, Grad Sch Engn, Div Mol Chem, Kita Ku, Kita 13,Nishi 8, Sapporo, Hokkaido 0608628, Japan Hokkaido Univ Kita 13,Nishi 8 Sapporo Hokkaido Japan 0608628 n
Citazione:
T. Kamidate et al., "Effect of the geometry of microfabricated flow reactors on chemiluminescent detection of epinephrine with lucigenin", LUMINESCENC, 16(5), 2001, pp. 337-342

Abstract

Three types of flow reactors with different lengths (12-126 mm) and widths(0.4-2.0 nun) of channel were made on the silicon chip by microfabricationtechniques for the chemiluminescent (CL) detection of epinephrine (EP) with lucigenin (Luc). The volume of each CL reactor was about 10 muL. A solution containing EP and Luc and a solution containing NaOH and periodate were injected successively into each inlet of the CL reactor in the range 20-100muL/min with a pressure-driven flow system. The intensity of light emission was dependent on the geometry of the flow reactors. These results could be explained in terms of the differences in the diffusion length of the reactants in the flow reactors. The maximum light emission were linearly correlated, with the concentrations of EP over the range from the detection lit-nit of 5.0 x 10(-8) mol/L up to 5.0 x 10(-6) mol/L on the use of the CL reactor with the most promising geometry. Copyright (C) 2001 John Wiley & Sons,Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:02:00