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Titolo:
In vivo optical imaging of expression of vascular endothelial growth factor following laser incision in skin
Autore:
Izzo, AD; Mackanos, MA; Beckham, JT; Jansen, ED;
Indirizzi:
Vanderbilt Univ, Dept Biomed Engn, Stn B, Nashville, TN 37235 USA Vanderbilt Univ Nashville TN USA 37235 gn, Stn B, Nashville, TN 37235 USA
Titolo Testata:
LASERS IN SURGERY AND MEDICINE
fascicolo: 4, volume: 29, anno: 2001,
pagine: 343 - 350
SICI:
0196-8092(2001)29:4<343:IVOIOE>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARBON-DIOXIDE LASER; FREE-ELECTRON LASER; GREEN FLUORESCENT PROTEIN; TISSUE INTERACTIONS; PROMOTER ACTIVITY; TENSILE-STRENGTH; PULSE DURATION; ORAL-MUCOSA; MU-M; ABLATION;
Keywords:
laser surgery; vascular endothelial growth factor (VEGF); free-electron laser; wound healing; fibroblast; cellular response; reporter gene; transgenic; mouse; thermal damage;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Jansen, ED Vanderbilt Univ, Dept Biomed Engn, Stn B, Box 79, Nashville, TN37235 USA Vanderbilt Univ Box 79 Nashville TN USA 37235 lle, TN 37235 USA
Citazione:
A.D. Izzo et al., "In vivo optical imaging of expression of vascular endothelial growth factor following laser incision in skin", LASER SURG, 29(4), 2001, pp. 343-350

Abstract

Background and Objective: Laser-tissue interaction studies have focused onlaser-induced secondary effects on tissue and the postmortem histological analysis of laser wounds. In this study we addressed wound healing and possible impairment of wound healing due to collateral tissue damage by in vivoimaging of gene expression. Study Design/Material and Methods: We used a transgenic mouse model containing a VEGF promoter driving a GFP reporter gene to image VEGF expression in vivo. Twenty-two mice received two full thickness incisions in the dorsalskin: one with the Free Electron Laser (lambda = 6.45 mum, 52.9 mJ/mm(2)) and one with a scalpel. Mice were imaged for GFP expression at 3 days, 1, 2, 3, and 4 weeks. Confocal microscopic imaging was performed at 2 weeks. Results: Peak GFP expression was seen at 2-3 weeks and was localized in fibroblasts. FEL lesions showed more total GFP expression than scalpel lesions but this was only statistically significant (P < 0.05) at 2 and 4 weeks. The full-width half-max (FWHM) of the GFP expression was always larger for the FEL lesion compared to the scalpel lesion but was only statistically significant (P < 0.05) at 2 and 3 weeks. At 2 weeks the extent of the GFP expression in the laser lesion was on average 55 mum beyond that seen in the scalpel lesion but correlated with the number of laser passes. Conclusions: Feasibility of using transgenic mice carrying photoactive reporter genes for studying cellular process of laser-inflicted wound repair in a noninvasive, in vivo manner was shown. GFP expression mediated by the VEGF promoter in fibroblast showed minimal impairment of wound healing due to the laser. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/10/20 alle ore 22:33:47