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Titolo:
Isolation and characterization of mammary cDNA differentially expressed inlethal milk mutation mice
Autore:
Bao, B; Lee, DY; Prasad, AS;
Indirizzi:
Wayne State Univ, Sch Med, Dept Internal Med, Detroit, MI 48201 USA Wayne State Univ Detroit MI USA 48201 Internal Med, Detroit, MI 48201 USA Warner Lambert Parke Davis, Parke Davis Pharmaceut Res, Ann Arbor, MI 48105 USA Warner Lambert Parke Davis Ann Arbor MI USA 48105 Ann Arbor, MI 48105 USA
Titolo Testata:
JOURNAL OF TRACE ELEMENTS IN EXPERIMENTAL MEDICINE
fascicolo: 4, volume: 14, anno: 2001,
pagine: 371 - 382
SICI:
0896-548X(2001)14:4<371:IACOMC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CASEIN GENE-EXPRESSION; ACQUIRED ZINC-DEFICIENCY; ACIDIC PROTEIN GENE; MESSENGER-RNA; EPITHELIAL-CELLS; SACCHAROMYCES-CEREVISIAE; HORMONAL-REGULATION; MOLECULAR-CLONING; DISPLAY; MOUSE;
Keywords:
lm mutation; zinc metabolism; mRNA differential display; Northern hybridization; mammary gland; brain; cDNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Bao, B Wayne State Univ, Sch Med, Dept Internal Med, Detroit, MI 48201 USAWayne State Univ Detroit MI USA 48201 l Med, Detroit, MI 48201 USA
Citazione:
B. Bao et al., "Isolation and characterization of mammary cDNA differentially expressed inlethal milk mutation mice", J TR EL EXP, 14(4), 2001, pp. 371-382

Abstract

Lethal milk (lm) mutation in C57BL/6J inbred mice results in the reductionof zinc content in the milk and causes fatal neonatal zinc deficiency. Zinc injection to lm pups after birth help, them to overcome severe neonatal zinc deficiency and to maintain the normal body growth of lm pups. Thus, lm mutation displays a specific effect on the zinc content of the milk during lactation. The decrease in zinc content of them milk is not due to the low body zinc status in lactating lm dams. Also, oral zinc supplementation in lactating lm dams does not correct the low concentration of zinc in the milk. These findings suggest that lm mutation results in the defective cellulartransport of zinc. The defective zinc transport might alter the expressionof mammary genes because cellular zinc plays a critical role in diverse cellular functions. To investigate the effect of lm mutation on the expression of mammary genes, the differentially expressed cDNAs in lm mammary gland (MG) were explored using the mRNA differential display (DD) gel. One of theisolated cDNAs (M3) was expressed in the lm MG two times greater than thatin the normal MG. The cloned partial M3 cDNA was shown to have the similarities to the unknown functional cDNAs in the yeast and human tissues. The M3 cDNA might have a common function in both lower and higher eukaryotes. The M3 mRNA is rich in the brain and the MG, but not in the liver and kidney. Hence, the M3 cDNA may have a certain function associated with the brain and the MG. More information regarding the cloned M3 cDNA might be provided by screening of the full length of M3 cDNA from cDNA library. J. Trace Elem. Exp. Med. 14:371-382, 2001. (C) 2001 Wiley-Liss. Inc.

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Documento generato il 30/09/20 alle ore 07:55:24