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Titolo:
Inflamed glomeruli-specific gene activation that uses recombinant adenovirus with the Cre/loxP system
Autore:
Yokoo, T; Ohashi, T; Utsunomiya, Y; Shiba, H; Shen, JS; Hisada, Y; Eto, Y; Kawamura, T; Hosoya, T;
Indirizzi:
Jikei Univ, Sch Med, Dept Internal Med, Div Nephrol & Hypertens,Minato Ku,Tokyo 1058461, Japan Jikei Univ Tokyo Japan 1058461 Hypertens,Minato Ku,Tokyo 1058461, Japan Jikei Univ, Sch Med, Inst DNA Med, Dept Gene Therapy, Tokyo 1058461, JapanJikei Univ Tokyo Japan 1058461 , Dept Gene Therapy, Tokyo 1058461, Japan Jikei Univ, Sch Med, Dept Paediat, Tokyo 1058461, Japan Jikei Univ TokyoJapan 1058461 h Med, Dept Paediat, Tokyo 1058461, Japan Tanabe Seiyaku Co Ltd, Discovery Res Lab, Osaka, Japan Tanabe Seiyaku Co Ltd Osaka Japan Ltd, Discovery Res Lab, Osaka, Japan
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
fascicolo: 11, volume: 12, anno: 2001,
pagine: 2330 - 2337
SICI:
1046-6673(200111)12:11<2330:IGGATU>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SITE-SPECIFIC RECOMBINATION; RAT MESANGIAL CELLS; SMOOTH MUSCLE ACTIN; CRE RECOMBINASE; MAMMALIAN-CELLS; VEHICLE CELLS; EXPRESSION; INTERLEUKIN-1; MICE; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Yokoo, T Jikei Univ, Sch Med, Dept Internal Med, Div Nephrol & Hypertens,Minato Ku,3-25-8 Nishi Shimbashi, Tokyo 1058461, Japan Jikei Univ 3-25-8 Nishi Shimbashi Tokyo Japan 1058461 461, Japan
Citazione:
T. Yokoo et al., "Inflamed glomeruli-specific gene activation that uses recombinant adenovirus with the Cre/loxP system", J AM S NEPH, 12(11), 2001, pp. 2330-2337

Abstract

The authors previously reported that bone marrow-derived CD11b(+)CD18(+) cells could be used as a vehicle to deliver foreign genes into inflamed glomeruli and that this vehicle cell (v-cell) could retard the progression of nephritis by delivering anti-inflammatory molecules. As a next step, the authors tried to establish a switching system by which v-cells are activated only at the inflamed glomeruli. A recombinant adenovirus (Ad) that expressedCre recombinase under the control of the interleukin-1 beta (IL-1 beta) promoter (AxIL-1pr/Cre) was constructed and transfected into v-cells. After confirming that AxIL-1pr/Cre expresses Cre by lipopolysaccharide (LPS) treatment, AxIL-1pr/Cre was infected together with another Ad bearing a switching reporter unit in which the LacZ gene is activated under the control of the CAG promoter by the Cre-mediated excisional deletion of interposed stuffer DNA. Only a negligible number of double-infected (Cre/loxPCAG) cells expressed LacZ. This number, however, was significantly increased by LPS, whichsuggests that LPS-induced Cre effectively deletes the stuffer DNA, which allows for a complete CAG promoter. DBA/2j mice were then transplanted with Cre/loxPCAG cells via a tail vein and treated with anti-glomerular basementmembrane (GBM) serum. To trace the transplanted cells, marker v-cells, infected with AxCANLacZ to constitutively express the LacZ gene, were also used. Although transplanted cells expressing LacZ collected in the spleen independent of anti-GBM treatment, they did not express the LacZ gene in the mice transplanted with Cre/loxPCAG cells. On the other hand, transplanted cells were recruited in the glomeruli and expressed the LacZ gene upon anti-GBM treatment. These results suggested that only the v-cells recruited in theglomeruli could be switched on and activate Foreign genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/08/20 alle ore 19:21:53