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Titolo:
Transient localization of gamma-tubulin around the centrioles in the nuclear division of Boergesenia forbesii (Siphonocladales, Chlorophyta)
Autore:
Motomura, T; Nagasato, C; Komeda, Y; Okuda, K;
Indirizzi:
Hokkaido Univ, Field Sci Ctr No Biosphere, Muroran Marine Stn, Muroran, Hokkaido 0510003, Japan Hokkaido Univ Muroran Hokkaido Japan 0510003 ran, Hokkaido 0510003, Japan Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Sapporo, Hokkaido 0600810, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600810 oro, Hokkaido 0600810, Japan Kochi Univ, Fac Sci, Dept Biol, Kochi 7808520, Japan Kochi Univ Kochi Japan 7808520 Fac Sci, Dept Biol, Kochi 7808520, Japan
Titolo Testata:
JOURNAL OF PHYCOLOGY
fascicolo: 5, volume: 37, anno: 2001,
pagine: 783 - 792
SICI:
0022-3646(200110)37:5<783:TLOGAT>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROTUBULE-ORGANIZING CENTERS; FUCUS-DISTICHUS FUCALES; MITOTIC SPINDLE POLES; XENOPUS EGG EXTRACTS; CELL-CYCLE; PERICENTRIOLAR MATERIAL; SELF-ORGANIZATION; ANIMAL-CELLS; PLANT-CELLS; MITOSIS;
Keywords:
Boergesenia forbesii; centrioles; centrosome; closed mitotic spindle; gamma-tubulin; immunofluorescence microscopy; MTOCs; spindle formation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Motomura, T Hokkaido Univ, Field Sci Ctr No Biosphere, Muroran Marine Stn,Muroran, Hokkaido 0510003, Japan Hokkaido Univ Muroran Hokkaido Japan 0510003 o 0510003, Japan
Citazione:
T. Motomura et al., "Transient localization of gamma-tubulin around the centrioles in the nuclear division of Boergesenia forbesii (Siphonocladales, Chlorophyta)", J PHYCOLOGY, 37(5), 2001, pp. 783-792

Abstract

Mitosis in Boergesenia forbesii (Harvey) Feldman was studied by immunofluorescence microscopy using anti-beta -tubulin, anti-gamma -tubulin, and anti-centrin antibodies. In the interphase nucleus, one, two, or rarely three anti-centrin staining spots were located around the nucleus, indicating the existence of centrioles. Microtubules (MTs) elongated randomly from the circumference of the nuclear envelope, but distinct microtubule organizing centers could not be observed. In prophase, MTs located around the interphase nuclei became fragmented and eventually disappeared. Instead, numerous MTs elongated along the nuclear envelope from the discrete anti-centrin staining spots. Anti-centrin staining spots duplicated and migrated to the two mitotic poles. gamma -Tubulin was not detected at the centrioles during interphase but began to localize there from prophase onward. The mitotic spindle in B. forbesii was a typical closed type, the nuclear envelope remaining intact during nuclear division. From late prophase, accompanying the chromosome condensation, spindle MTs could be observed within the nuclear envelope. A bipolar mitotic spindle was formed at metaphase, when the most intense staining of gamma -tubulin around the centrioles could also be seen. Both spindle MT poles were formed inside the nuclear envelope, independent of the position of the centrioles outside. In early anaphase, MTs between separating daughter chromosomes were not detected. Afterward, characteristic interzonal spindle MTs developed and separated both sets of the daughter chromosomes. From late anaphase to telophase, gamma -tubulin could not be detected around the centrioles and MT radiation from the centrioles became diminished at both poles. gamma -Tubulin was not detected at the ends of the interzonal spindle fibers. When MTs were depolymerized with amiprophos methyl during mitosis, gamma -tubulin localization around the centrioles was clearly confirmed. Moreover, an influx of tubulin molecules into the nucleus for themitotic spindle occurred at chromosome condensation in mitosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:15:06