Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A reversed-phase thin-layer chromatography/scanning densitometric method for the analysis of gardenia yellow in food using crocetin as an indicator
Autore:
Ozeki, N; Oka, H; Ito, Y; Ueno, E; Goto, T; Hayashi, T; Itakura, Y; Ito, T; Maruyama, T; Turuta, M; Miyazawa, T; Matsumoto, H;
Indirizzi:
Aichi Prefectural Inst Publ Hlth, Kita Ku, Nagoya, Aichi 4628576, Japan Aichi Prefectural Inst Publ Hlth Nagoya Aichi Japan 4628576 628576, Japan Seto Publ Hlth Ctr Aichi Prefecture, Aichi 4890808, Japan Seto Publ Hlth Ctr Aichi Prefecture Aichi Japan 4890808 i 4890808, Japan Kasugai Publ Hlth Ctr Aichi Prefecture, Aichi 4860927, Japan Kasugai Publ Hlth Ctr Aichi Prefecture Aichi Japan 4860927 860927, Japan Okazaki Publ Hlth Ctr Aichi Prefecture, Aichi 4448545, Japan Okazaki Publ Hlth Ctr Aichi Prefecture Aichi Japan 4448545 448545, Japan
Titolo Testata:
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
fascicolo: 18, volume: 24, anno: 2001,
pagine: 2849 - 2860
SICI:
1082-6076(2001)24:18<2849:ARTCDM>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL COLORING MATTERS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Oka, H Aichi Prefectural Inst Publ Hlth, Kita Ku, 7-6 Nagare, Nagoya, Aichi 4628576, Japan Aichi Prefectural Inst Publ Hlth 7-6 Nagare Nagoya Aichi Japan 4628576
Citazione:
N. Ozeki et al., "A reversed-phase thin-layer chromatography/scanning densitometric method for the analysis of gardenia yellow in food using crocetin as an indicator", J LIQ CHR R, 24(18), 2001, pp. 2849-2860

Abstract

In the present study, a TLC method for the analysis of gardenia yellow in foods using crocetin as an indicator was developed. Gardenia yellow was extracted from food samples with methanol or hydrous methanol, and after the extract was evaporated, the residue was dissolved in water and the pH was adjusted to 11 or above with 1 mol/L NaOH. The resultant mixture was occasionally stirred, then allowed to stand in a water bath at 50 degreesC for 30 minutes. Subsequently, the pH of the mixture was slightly acidified using hydrochloric acid. It was then purified through a C18 cartridge before being subjected to the TLC analysis. The TLC conditions were as follows: plate, RP-18F254S (Art. 15389, E. Merck); solvent system, acetonitrile-tetrahydrofuran-0.1 mol/L oxalic acid (7:8:7). The visible absorption spectrum of the color was measured using scanning densitometry without isolation of the color. In order to investigate thecapability of the present method, 37 commercial foods were analyzed, and their chromatographic behaviors and spectra were observed. The separation and the spectra obtained were not affected by coexisting substances in the foods, and the spots always gave the same Rf values and spectra as the standard, along with good reproducibility. The present method is considered to be useful for the rapid analysis of gardenia yellow in foods.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 20:39:15