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Titolo:
Recombinant Escherichia coli engineered for production of L-lactic acid from hexose and pentose sugars
Autore:
Dien, BS; Nichols, NN; Bothast, RJ;
Indirizzi:
USDA ARS, Natl Ctr Agr Utilizat Res, Fermentat Biochem Res Unit, Peoria, IL 61604 USA USDA ARS Peoria IL USA 61604 entat Biochem Res Unit, Peoria, IL 61604 USA
Titolo Testata:
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
fascicolo: 4, volume: 27, anno: 2001,
pagine: 259 - 264
SICI:
1367-5435(200110)27:4<259:RECEFP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
FERMENTATIVE LACTATE-DEHYDROGENASE; STREPTOCOCCUS-BOVIS; ETHANOL-PRODUCTION; GENE; CLONING; PH; COMPLEMENTATION; INHIBITION; TOXICITY; SEQUENCE;
Keywords:
lactic acid production; Escherichia coli; fermentation; pentoses;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Dien, BS USDA ARS, Natl Ctr Agr Utilizat Res, Fermentat Biochem Res Unit, 1815 N Univ St, Peoria, IL 61604 USA USDA ARS 1815 N Univ St Peoria IL USA 61604 Peoria, IL 61604 USA
Citazione:
B.S. Dien et al., "Recombinant Escherichia coli engineered for production of L-lactic acid from hexose and pentose sugars", J IND MIC B, 27(4), 2001, pp. 259-264

Abstract

Recombinant Escherichia coli have been constructed for the conversion of glucose as well as pentose sugars into L-lactic acid. The strains carry the lactate dehydrogenase gene from Streptococcus bovis on a low copy number plasmid for production of L-lactate. Three E. coli strains were transformed with the plasmid for producing L-lactic acid. Strains FBR9 and FBR11 were serially transferred 10 times in anaerobic cultures in sugar-limited medium containing glucose or xylose without selective antibiotic. An average of 96%of both FBR9 and FBR11 cells maintained pVALDH1 in anaerobic cultures. Thefermentation performances of FBR9, FBR10, and FBR11 were compared in pH-controlled batch fermentations with medium containing 10% w/v glucose. Fermentation results were superior for FBR11, an E. coli B strain, compared to those observed for FBR9 or FBR10. FBR11 exhausted the glucose within 30 h, and the maximum lactic acid concentration (7.32% w/v) was 93% of the theoretical maximum. The other side-products detected were cell mass and succinic acid (0.5 g/l).

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Documento generato il 09/04/20 alle ore 07:47:39