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Titolo:
Dynamic change of cells expressing MMP-2 mRNA and MT1-MMP mRNA in the recovery from liver fibrosis in the rat
Autore:
Watanabe, T; Niioka, M; Ishikawa, A; Hozawa, S; Arai, M; Maruyama, K; Okada, A; Okazaki, I;
Indirizzi:
Tokai Univ, Sch Med, Dept Environm Hlth, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 sehara, Kanagawa 2591193, Japan Tokai Univ, Sch Med, Dept Community Hlth, Kanagawa, Japan Tokai Univ Kanagawa Japan Sch Med, Dept Community Hlth, Kanagawa, Japan Hiratsuka City Hosp, Dept Internal Med, Kanagawa, Japan Hiratsuka City Hosp Kanagawa Japan , Dept Internal Med, Kanagawa, Japan Kurihama Natl Hosp, Dept Internal Med, Kanagawa, Japan Kurihama Natl HospKanagawa Japan p, Dept Internal Med, Kanagawa, Japan Japan Sci & Technol Corp, Matrix Signaling Project, Exploratory Res Adv Technol, Aichi, Japan Japan Sci & Technol Corp Aichi Japan tory Res Adv Technol, Aichi, Japan
Titolo Testata:
JOURNAL OF HEPATOLOGY
fascicolo: 4, volume: 35, anno: 2001,
pagine: 465 - 473
SICI:
0168-8278(200110)35:4<465:DCOCEM>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATIC STELLATE CELLS; MATRIX METALLOPROTEINASE-2 ACTIVATION; GENE-EXPRESSION; IV COLLAGENASE; MEMBRANE-TYPE-1; FIBROGENESIS; INHIBITORS; METASTASIS; GROWTH; P53;
Keywords:
hepatic stellate cell; matrix metalloproteinase; matrix metalloproteinase 2; membrane type 1 matrix metalloproteinase; reverse transcription-polymerase chain reaction; in situ hybridization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Watanabe, T Tokai Univ, Sch Med, Dept Environm Hlth, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 gawa 2591193, Japan
Citazione:
T. Watanabe et al., "Dynamic change of cells expressing MMP-2 mRNA and MT1-MMP mRNA in the recovery from liver fibrosis in the rat", J HEPATOL, 35(4), 2001, pp. 465-473

Abstract

Background/Aims: The aim of this study was to investigate whether both matrix metalloproteinase-2 (MMP-2) and membrane type 1 MMP (MT1-MMP) participate in the spontaneous resolution of liver fibrosis. Methods: Transcription of both genes was examined by reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH). Gelatinase activity was investigated by zymography. Results: Gene expression by RT-PCR showed that both genes increased in theprocess of liver fibrosis, then decreased gradually in the recovery phase. ISH revealed that distribution of positive cells changed quickly in the recovery phase. Positive cells were widely seen in the liver, mainly around fibrous septa, in the aggressive phase, but were exclusively observed at theinterface between the resolving fibrous band and the parenchyma, then werediffusely located in the lobules in the recovery phase. Main cells expressing both mRNAs seemed to be stellate cells for their morphology, though they did not express characteristic cell markers. Some hepatocytes and Kupffercells expressed both mRNAs in the recovery phase. Gelatinase activity of MMP-2 increased in the recovery phase of 8-week-treated rat liver by gelatinzymography. Conclusions: The results of present study suggest that both enzymes participate in the destruction of extracellular matrix in coordination with MMP-13. (C) 2001 European Association for the Study of the Liver. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 18:44:17