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Titolo:
High-performance liquid chromatographic analysis for the determination of a novel polymer-bound camptothecin derivative (MAG-camptothecin) and free camptothecin in human plasma
Autore:
Schoemaker, NE; Frigerio, E; Fraier, D; Schellens, JHM; Rosing, H; Jansen, S; Beijnen, JH;
Indirizzi:
Netherlands Canc Inst, Slotervaart Hosp, Dept Pharm & Pharmacol, NL-1066 EC Amsterdam, Netherlands Netherlands Canc Inst Amsterdam Netherlands NL-1066 EC rdam, Netherlands Netherlands Canc Inst, Dept Med Oncol, Antoni Van Leeuwenhoek Hosp, NL-1066 CX Amsterdam, Netherlands Netherlands Canc Inst Amsterdam Netherlands NL-1066 CX rdam, Netherlands Univ Utrecht, Fac Pharm, Div Drug Toxicol, NL-3584 CA Utrecht, NetherlandsUniv Utrecht Utrecht Netherlands NL-3584 CA 3584 CA Utrecht, Netherlands Pharmacia & Upjohn Inc, Drug Metab Res, Dept Metab & Pharmacokinet, I-20014 Milan, Italy Pharmacia & Upjohn Inc Milan Italy I-20014 cokinet, I-20014 Milan, Italy
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 763, anno: 2001,
pagine: 173 - 183
SICI:
1387-2273(20011105)763:1-2<173:HLCAFT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYETHYLENE-GLYCOL; DELIVERY SYSTEMS; CONJUGATION; EFFICACY;
Keywords:
camptothecin; MAG-camptothecin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Schoemaker, NE Netherlands Canc Inst, Slotervaart Hosp, Dept Pharm & Pharmacol, Louwesweg6, NL-1066 EC Amsterdam, Netherlands Netherlands Canc Inst Louwesweg 6 Amsterdam Netherlands NL-1066 EC
Citazione:
N.E. Schoemaker et al., "High-performance liquid chromatographic analysis for the determination of a novel polymer-bound camptothecin derivative (MAG-camptothecin) and free camptothecin in human plasma", J CHROMAT B, 763(1-2), 2001, pp. 173-183

Abstract

A selective HPLC assay is described for the determination of free and total (free plus polymer-bound) camptothecin (CPT) in human plasma after administration of the anti-tumor drug MAG-CPT (polymer bound camptothecin). TotalCPT levels were determined after hydrolysis and free CPT was extracted from acidified plasma using Oasis solid-phase extraction material. Extracts were analyzed on a Zorbax SB-C-8 analytical column, using a mixture of acetonitrile-25 mM phosphate buffer (pH 4.0) as the eluent. Detection was performed fluorimetrically. Concentrations of polymer-bound CPT were calculated bysubtraction of free from total CPT. The lower limits of quantitation of the methods were 100 ng/ml for total and 1.0 ng/ml for free CPT using 50 mul and 250 mul plasma, respectively. Special attention was paid to the stability of the analytes. The presented method was successfully applied in a clinical pharmacokinetic study in our institute. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:55:29