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Titolo:
Development of a high-performance liquid chromatographic method for bioanalytical applications with sulpiride
Autore:
Huang, MC; Ho, HO; Yeh, GC; Ke, WT; Lin, LC; Hsu, TMB; Kao, CC; Sheu, MT;
Indirizzi:
Taipei Med Univ, Grad Inst Pharmaceut Sci, Taipei 110, Taiwan Taipei Med Univ Taipei Taiwan 110 nst Pharmaceut Sci, Taipei 110, Taiwan Taipei Med Univ, Dept Pediat, Taipei 110, Taiwan Taipei Med Univ Taipei Taiwan 110 Univ, Dept Pediat, Taipei 110, Taiwan Taiwan Biopharmtech Co Ltd, Taipei, Taiwan Taiwan Biopharmtech Co Ltd Taipei Taiwan armtech Co Ltd, Taipei, Taiwan Sintong Chem Ind Co Ltd, Tao Yuan, Taiwan Sintong Chem Ind Co Ltd Tao Yuan Taiwan em Ind Co Ltd, Tao Yuan, Taiwan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 763, anno: 2001,
pagine: 157 - 163
SICI:
1387-2273(20011105)763:1-2<157:DOAHLC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE DETECTION; QUANTITATIVE-ANALYSIS; HUMAN-PLASMA; PHARMACOKINETICS; SEPARATION; DRUGS;
Keywords:
silica column; reversed phase eluents; bioequivalence; sulpiride;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Sheu, MT Taipei Med Univ, Grad Inst Pharmaceut Sci, 250 Wu Hsing St, Taipei 110, Taiwan Taipei Med Univ 250 Wu Hsing St Taipei Taiwan 110 ei 110, Taiwan
Citazione:
M.C. Huang et al., "Development of a high-performance liquid chromatographic method for bioanalytical applications with sulpiride", J CHROMAT B, 763(1-2), 2001, pp. 157-163

Abstract

An improved HPLC method using a silica gel column with fluorescence detection (excitation at 300 nm and emission at 365 nm) was developed for the determination of sulpiride concentrations in plasma. Analysis of sulpiride in plasma samples was simplified by a one-step Liquid-liquid extraction after alkaline treatment of only 1 ml of plasma. The low limit of quantitation was 20 ng/ml with a coefficient of variation of less than 20%. A linear rangewas found from 20 to 1500 ng/ml This HPLC method was validated with the precision for inter-day and intra-day runs being 0.36-8.01% and 0.29-5.25%, respectively, and the accuracy (standard deviation of mean, SD) for inter-day and intra-day runs being -1.58 to 5.02% and -2.14 to 5.21%, respectively. Bioequivalence of the two products was evaluated in 12 normal healthy malevolunteers in a single-dose, two-period, two-sequence, two-treatment cross-over study. Sulpiride plasma concentrations were analyzed with this validated HPLC method. Results demonstrated that the two tablet formulations of sulpiride appear to be bioequivalent. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 03:23:22