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Titolo:
Sensitive liquid chromatographic assay for the basic DNA intercalator (N,N-dimethylaminoethyl)-9-amino-5-methylacridine-4-carboxamide and its nitroarylmethyl quaternary prodrugs in biological samples
Autore:
Ferry, DM; van Zijl, PL; Wilson, WR;
Indirizzi:
Univ Auckland, Fac Med & Hlth Sci, Canc Soc Res Ctr, Auckland 1, New Zealand Univ Auckland Auckland New Zealand 1 oc Res Ctr, Auckland 1, New Zealand
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 763, anno: 2001,
pagine: 149 - 156
SICI:
1387-2273(20011105)763:1-2<149:SLCAFT>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYPOXIA-SELECTIVE CYTOTOXINS; ANTITUMOR AGENTS; SALTS; MECHLORETHAMINE; MITOXANTRONE; REDUCTION; RADIATION;
Keywords:
DNA intercalators; N,N-dimethylaminoethyl-9-amino-5-methylacridine-4-carboxamide; nitroarylmethyl quaternary salts;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Wilson, WR Univ Auckland, Fac Med & Hlth Sci, Canc Soc Res Ctr, Private Bag 92019, Auckland 1, New Zealand Univ Auckland Private Bag 92019 Auckland New Zealand 1 Zealand
Citazione:
D.M. Ferry et al., "Sensitive liquid chromatographic assay for the basic DNA intercalator (N,N-dimethylaminoethyl)-9-amino-5-methylacridine-4-carboxamide and its nitroarylmethyl quaternary prodrugs in biological samples", J CHROMAT B, 763(1-2), 2001, pp. 149-156

Abstract

Nitroarylmethyl quaternary (NMQ) ammonium salts of the basic DNA intercalator AMAC (N,N-dimethylaminoethyl-9-amino-5-methylacridine-4-carboxamide) are of interest as anticancer prodrugs. A sensitive HPLC assay has been developed for quantitation of AMAC and its NMQ prodrugs in cultured cells, plasma and tissue. Recovery of the prodrugs, without conversion to AMAC, was achieved using extraction in alkaline acetonitrile followed by immediate reneutralisation. Reversed-phase HPLC with fluorescence detection gave a detection limit of 3 fmol for AMAC, with linearity to 20 nmol (using diode array absorbance at high concentrations). This assay was used to measure cellular uptake, and hypoxic metabolism to AMAC, of three NMQ-AMAC prodrugs. (C) 2001 Elsevier Science B.V. All. rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 13:45:26