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Titolo:
Discontinuous electrophoretic stacking system for cholate-based electrokinetic chromatographic separation of 8-hydroxy-2 '-deoxyguanosine from unmodified deoxynucleosides
Autore:
Strein, TG; Morris, D; Palmer, J; Landers, JP;
Indirizzi:
Univ Virginia, Dept Chem, Charlottesville, VA 22901 USA Univ Virginia Charlottesville VA USA 22901 Charlottesville, VA 22901 USA Bucknell Univ, Dept Chem, Lewisburg, PA 17837 USA Bucknell Univ LewisburgPA USA 17837 , Dept Chem, Lewisburg, PA 17837 USA Rose Hulman Inst Technol, Dept Chem, Terre Haute, IN 47803 USA Rose HulmanInst Technol Terre Haute IN USA 47803 rre Haute, IN 47803 USA Univ Virginia, Hlth Sci Ctr, Dept Pathol, Charlottesville, VA 22901 USA Univ Virginia Charlottesville VA USA 22901 Charlottesville, VA 22901 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 763, anno: 2001,
pagine: 71 - 78
SICI:
1387-2273(20011105)763:1-2<71:DESSFC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDATIVE DNA-DAMAGE; PERFORMANCE LIQUID-CHROMATOGRAPHY; CAPILLARY-ELECTROPHORESIS; NEUTRAL ANALYTES; ELECTROCHEMICAL DETECTION; MODIFIED NUCLEOSIDES; HUMAN-DISEASE; 8-HYDROXYDEOXYGUANOSINE; NUCLEOTIDES; MATRICES;
Keywords:
stacking; 8-hydroxy-2 '-deoxyguanosine; DNA damage; UV detection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Landers, JP Univ Virginia, Dept Chem, Mccormick Rd, Charlottesville, VA 22901 USA Univ Virginia Mccormick Rd Charlottesville VA USA 22901 01 USA
Citazione:
T.G. Strein et al., "Discontinuous electrophoretic stacking system for cholate-based electrokinetic chromatographic separation of 8-hydroxy-2 '-deoxyguanosine from unmodified deoxynucleosides", J CHROMAT B, 763(1-2), 2001, pp. 71-78

Abstract

The stacking and baseline-resolved separation of the oxidative damage marker, 8-hydroxy-2'-deoxyguanosine (8-OHdG), from unmodified deoxynucleosides in under 4 min is reported. Separations of 8-OHdG from 2'-deoxyadenosine, 2'-deoxycytosine, 2'-deoxyguanosine, and thymidine are accomplished using micellar electrokinetic capillary chromatography with sodium cholate. Importantly, the use of sulfate, intentionally added to the sample matrix, resultsin effective stacking of 8-OHdG and other analytes. This work extends electrokinetic stacking injection of neutral analytes to include deoxynucleosides, The procedure works well with either electrokinetic or hydrodynamic injection. The separation buffer and sample matrix composition were optimized to effect stacking conditions with an uncoated 50 mum fused-silica capillary. The lower limit of detection for the analytes is in the nanomolar range,and is more than an order of magnitude lower than without stacking. With 30 s (5.7 cm) electrokinetic injections, stacking and baseline separation of8-hydroxy-2'-deoxyguanosine from the unmodified nucleosides is accomplished, even in the presence of a 400-fold excess of unmodified deoxynueleosides. (C) 2001 Published by Elsevier Science B.V.

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Documento generato il 05/04/20 alle ore 05:38:05