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Titolo:
Quantitation of promethazine and metabolites in urine samples using on-line solid-phase extraction and column-switching
Autore:
Song, Q; Putcha, L;
Indirizzi:
NASA, Lyndon B Johnson Space Ctr, Life Sci Res Labs, Houston, TX 77058 USANASA Houston TX USA 77058 e Ctr, Life Sci Res Labs, Houston, TX 77058 USA Wyle Labs, Life Sci Syst & Serv, Houston, TX 77058 USA Wyle Labs Houston TX USA 77058 ife Sci Syst & Serv, Houston, TX 77058 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 763, anno: 2001,
pagine: 9 - 20
SICI:
1387-2273(20011105)763:1-2<9:QOPAMI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; ONLINE TRACE-ENRICHMENT; HUMAN-PLASMA; ELECTROCHEMICAL DETECTION; MASS-SPECTROMETRY; FLUORESCENCE DETECTION; RETINOIC ACID; HUMAN SERUM; INJECTION; SORBENTS;
Keywords:
column switching; promethazine; promethazine sulfoxide; monodesmethylpromethazine sulfoxide; monodesmethyl promethazine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Putcha, L NASA, Lyndon B Johnson Space Ctr, Life Sci Res Labs, Mail Code SD3, Houston, TX 77058 USA NASA Mail Code SD3 Houston TX USA 77058 3, Houston, TX 77058 USA
Citazione:
Q. Song e L. Putcha, "Quantitation of promethazine and metabolites in urine samples using on-line solid-phase extraction and column-switching", J CHROMAT B, 763(1-2), 2001, pp. 9-20

Abstract

A chromatographic method for the quantitation of promethazine (PMZ) and its three metabolites in urine employing on-line solid-phase extraction and column-switching has been developed. The column-switching system described here uses an extraction column for the purification of PMZ and its metabolites from a urine matrix, The extraneous matrix interference was removed by flushing the extraction column with a gradient elution. The analytes of interest were then eluted onto an analytical column for further chromatographicseparation using a mobile phase of greater solvent strength. This method is specific and sensitive with a range of 3.75-1400 ng/ml for PMZ and 2.5-1400 ng/ml for the metabolites promethazine sulfoxide, monodesmethyl promethazine sulfoxide and monodesmethyl promethazine. The lower limits of quantitation (LLOQ) were 3.75 ng/ml with less than 6.2% CV for PMZ and 2.50 ng/ml with less than 11.5% CV for metabolites based on a signal-to-noise ratio of 10:1 or greater. The accuracy and precision were within +/- 11.8% in bias and not greater than 5.5% CV in intra- and inter-assay precision for PMZ andmetabolites. Method robustness was investigated using a Plackett-Bunnan experimental design. The applicability of the analytical method for pharmacokinetic studies in humans is illustrated. (C) 2001 Elsevier Science B.V. Allrights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:40:35