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Titolo:
Simultaneous determination of retinol, beta-carotene and alpha-tocopherol in adipose tissue by high-performance liquid chromatography
Autore:
Casal, S; Macedo, B; Oliveira, MBPP;
Indirizzi:
Fac Farm Porto, Serv Bromatol, CEQUP, P-4050047 Oporto, Portugal Fac Farm Porto Oporto Portugal P-4050047 QUP, P-4050047 Oporto, Portugal
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 763, anno: 2001,
pagine: 1 - 8
SICI:
1387-2273(20011105)763:1-2<1:SDORBA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SERUM; FATTY-ACIDS; PLASMA; LYCOPENE; ANTIOXIDANTS; WOMEN; FOOD;
Keywords:
vitamins; retinol; beta-carotene; alpha-tocopherol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Casal, S Fac Farm Porto, Serv Bromatol, CEQUP, Rua Anibal Cunha,164, P-4050047 Oporto, Portugal Fac Farm Porto Rua Anibal Cunha,164 Oporto Portugal P-4050047 al
Citazione:
S. Casal et al., "Simultaneous determination of retinol, beta-carotene and alpha-tocopherol in adipose tissue by high-performance liquid chromatography", J CHROMAT B, 763(1-2), 2001, pp. 1-8

Abstract

A method is described for evaluation of fat-soluble vitamin in human adipose tissue with the aim to obtain, accurately and within the shortest analysis time, a time-integrated measure of exposure to vitamins from the diet. Fat tissue was deproteinized with ethanol and extracted with n-hexane. Normal-phase HPLC was performed in a Lichrosorb Si60 column with a gradient of n-hexane-2-propanol at 1 ml/min. Detection was accomplished using a diode-array system (for retinol and beta -carotene) in series with a fluorescence detector (alpha -tocopherol). The method was validated and applied to human adipose tissue in a total of 140 subjects. The mean contents found were 0.43, 0.84, 240.3 mug/g for retinol, beta -carotene and alpha -tocopherol, respectively. The method is sensitive enough for detecting the compounds in 1.6 mg of adipose tissue considering the lowest concentration found. (C) 2001Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:20:41