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Titolo:
Practical method for evaluation of linearity and effective pathlength of on-capillary photometric detectors in capillary electrophoresis
Autore:
Johns, C; Macka, M; Haddad, PR; King, M; Paull, B;
Indirizzi:
Univ Tasmania, Sch Chem, Hobart, Tas 7001, Australia Univ Tasmania HobartTas Australia 7001 Chem, Hobart, Tas 7001, Australia Dublin City Univ, Sch Chem Sci, Natl Ctr Sensor Res, Dublin 9, Ireland Dublin City Univ Dublin Ireland 9 Natl Ctr Sensor Res, Dublin 9, Ireland
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 927, anno: 2001,
pagine: 237 - 241
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROBES; ANIONS; DYES;
Keywords:
detection; electrophoresis; photometric detection; indirect detection; instrumentation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Haddad, PR Univ Tasmania, Sch Chem, GPO Box 252-75, Hobart, Tas 7001, Australia Univ Tasmania GPO Box 252-75 Hobart Tas Australia 7001 ustralia
Citazione:
C. Johns et al., "Practical method for evaluation of linearity and effective pathlength of on-capillary photometric detectors in capillary electrophoresis", J CHROMAT A, 927(1-2), 2001, pp. 237-241

Abstract

The optical characteristics of on-capillary photometric detectors for capillary electrophoresis were evaluated and five commercial detectors were compared. Plots of sensitivity (absorbance/concentration) versus absorbance obtained with a suitable testing solution yield both the linear range and theeffective path length of the detector. The detector linearity is a crucialparameter when using absorbing electrolytes, such as for indirect photometric detection, and especially for highly absorbing electrolyte probe ions. The upper limits of the linear ranges (determined as 5% decline in sensitivity) for five commercial detectors ranged from 0.175 to 1.2 AU. The effective pathlength reflects the quality of the optical design of the detector and is equal to the capillary internal diameter only for a light beam passingexactly through the capillary centre, but becomes progressively shorter for imperfect optical designs. The determined effective pathlength for the five investigated detectors ranged from 49.7 to 64.6 mum for a 75 mum I.D. capillary. (C) 2001 Elsevier Science BM All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 18:47:28