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Titolo:
Noradrenaline and alpha-adrenergic signaling induce the hsp70 gene promoter in mollusc immune cells
Autore:
Lacoste, A; De Cian, MC; Cueff, A; Poulet, SA;
Indirizzi:
Univ Paris 06, CNRS, INSU, Stn Biol Roscoff, F-29682 Roscoff, France Univ Paris 06 Roscoff France F-29682 ol Roscoff, F-29682 Roscoff, France
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 19, volume: 114, anno: 2001,
pagine: 3557 - 3564
SICI:
0021-9533(200110)114:19<3557:NAASIT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEINS; OYSTER CRASSOSTREA-GIGAS; INDUCED STRESS-RESPONSE; AGE-DEPENDENT RESPONSE; MYTILUS-GALLOPROVINCIALIS; PACIFIC OYSTER; IN-VITRO; EXPRESSION; KINASE; ACTIVATION;
Keywords:
mollusc; immune cell; noradrenaline; heat shock protein; alpha-adrenergic signaling; thermotolerance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Lacoste, A Univ Paris 06, CNRS, INSU, Stn Biol Roscoff, Pl Georges Teissier,BP 74, F-29682 Roscoff, France Univ Paris 06 Pl Georges Teissier,BP 74 Roscoff France F-29682
Citazione:
A. Lacoste et al., "Noradrenaline and alpha-adrenergic signaling induce the hsp70 gene promoter in mollusc immune cells", J CELL SCI, 114(19), 2001, pp. 3557-3564

Abstract

Expression of heat shock proteins (hsp) is a homeostatic mechanism inducedin both prokaryotic and eukaryotic cells in response to metabolic and environmental insults. A growing body of evidence suggests that in mammals, thehsp response is integrated with physiological responses through neuroendocrine signaling. In the present study, we have examined the effect of noradrenaline (NA) on the hsp70 response in mollusc immune cells. Oyster and abalone hemocytes transfected with a gene construct containing a gastropod hsp70 gene promoter linked to the luciferase reporter-gene were exposed to physiological concentrations of NA, or to various alpha- and beta -adrenoceptoragonists and antagonists. Results show that NA and alpha -adrenergic stimulations induced the expression of luciferase in transfected mollusc immunocytes. Furthermore, exposure of hemocytes to NA or to the alpha -adrenoceptor agonist phenylephrine (PE) resulted in the expression of the inducible isoform of the hsp70 protein. Pertussis toxin (PTX), the phospholipase C (PLC) inhibitor U73122, the protein kinase C (PKC) inhibitor calphostin C, the Ca2+-dependent PKC inhibitor Go 6976 and the phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor LY294002 blocked the PE-mediated induction of the hsp70 gene promoter. These results suggest that alpha -adrenergic signaling induces the transcriptionnal upregulation of hsp70 in mollusc hemocytes through a PTX-sensitive G-protein, PLC, Ca2+-dependent PKC and PI 3-kinase. Thus, a functional link exists between neuroendocrine signaling and the hsp70response in mollusc immune cells.

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Documento generato il 12/07/20 alle ore 05:23:18