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Titolo:
In vivo evaluation of ammonia plasma modified ePTFE grafts for small diameter blood vessel replacement - A preliminary report
Autore:
Sipehia, R; Liszkowski, M; Lu, A;
Indirizzi:
McGill Univ, Fac Med, Artificial Cells & Organs Res Ctr, Montreal, PQ H3G 1Y6, Canada McGill Univ Montreal PQ Canada H3G 1Y6 Ctr, Montreal, PQ H3G 1Y6, Canada
Titolo Testata:
JOURNAL OF CARDIOVASCULAR SURGERY
fascicolo: 4, volume: 42, anno: 2001,
pagine: 537 - 542
SICI:
0021-9509(200108)42:4<537:IVEOAP>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-ENDOTHELIAL-CELLS; GROWTH-FACTOR PRODUCTION; VASCULAR GRAFTS; DACRON GRAFTS; CANINE MODEL; FIBRONECTIN; SURFACES; SERUM; BIOMATERIALS; ADSORPTION;
Keywords:
blood vessel prosthesis; polytetrafluoroethylene; ammonia; endothelium, vascular;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Sipehia, R McGill Univ, Fac Med, Artificial Cells & Organs Res Ctr, 3655 Drummond, Montreal, PQ H3G 1Y6, Canada McGill Univ 3655 Drummond Montreal PQCanada H3G 1Y6 Y6, Canada
Citazione:
R. Sipehia et al., "In vivo evaluation of ammonia plasma modified ePTFE grafts for small diameter blood vessel replacement - A preliminary report", J CARD SURG, 42(4), 2001, pp. 537-542

Abstract

Background Today, saphenous veins are most frequently used to reconstruct occluded or diseased small diameter vessels (less than or equal to6 min in diameter). However, these veins are unavailable in 30-40% of patients. In such a situation, prosthetic grafts provide the only alternative. Since an endothelial cell (EC) lining, important for maintaining a "haemostatic-thrombotic" balance, does not develop onto the intima of implanted grafts in humans, these grafts occlude within a short period of time. The failure of vascular grafts is attributed to their characteristics which are nonconducive towards endothelial cell adhesion, spreading and growth. In order to examine whether the patency of vascular grafts can be improved by the surface modification of grafts' intima, small diameter grafts were modified by a novelammonia plasma treatment to enhance their interactions with EC. Methods. Through laparotomy, ammonia plasma treated ePTFE (4 nun in diameter) grafts (n=3) and control untreated grafts (n=6) were implanted into thedistal infrarenal aorta of rabbits. At appropriate time, grafts and adherent tissue were removed, fixed, stained and embedded in Poly/Bed 812. Light microscopic examination of thin sections cut from the proximal and distal anastomatic and midportion segments of explanted grafts was carried outResults. In control group studies, all animals developed lower limb paraplegia within seven days of implantation. Light microscopic examination of explanted control grafts showed that control grafts were obstructed by thrombosis/intimal hyperplasia. However, ammonia plasma modified explanted grafts, after one month of transplantation, revealed an endothelial cell-like lining that covered the grafts' inner surfaces. Accordingly, these grafts remained patent in animals. Conclusions. The grafts' surfaces that are made conducive to EC adhesion and proliferation and host response may influence endothelial regeneration. it is hoped that the combination of angiogenic molecules (ie. endothelial cell specific growth factors such as VEGF) with ammonia plasma modified grafts may provide further insight into the development of ideal small diameterprosthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:17:05