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Titolo:
Genome and hormones: Gender differences in physiology - Selected contribution: Estrogen receptor-alpha gene transfer inhibits proliferation and NF-kappa B activation in VSM cells from female rats
Autore:
Sharma, RV; Gurjar, MV; Bhalla, RC;
Indirizzi:
Univ Iowa, Coll Med, Dept Anat & Cell Biol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 nat & Cell Biol, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Ctr Cardiovasc, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 Ctr Cardiovasc, Iowa City, IA 52242 USA
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 5, volume: 91, anno: 2001,
pagine: 2400 - 2406
SICI:
8750-7587(2001)91:5<2400:GAHGDI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; CORONARY HEART-DISEASE; REPLACEMENT THERAPY; CARDIOVASCULAR-SYSTEM; POSTMENOPAUSAL WOMEN; PREMENOPAUSAL WOMEN; BALLOON INJURY; DNA-BINDING; EXPRESSION; ATHEROSCLEROSIS;
Keywords:
vascular smooth muscle cells; nuclear factor-kappa B; Fischer 344 aged female rats;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Bhalla, RC Univ Iowa, Coll Med, Dept Anat & Cell Biol, Iowa City, IA 52242USA Univ Iowa Iowa City IA USA 52242 Biol, Iowa City, IA 52242 USA
Citazione:
R.V. Sharma et al., "Genome and hormones: Gender differences in physiology - Selected contribution: Estrogen receptor-alpha gene transfer inhibits proliferation and NF-kappa B activation in VSM cells from female rats", J APP PHYSL, 91(5), 2001, pp. 2400-2406

Abstract

Epidemiological studies have demonstrated that hormone replacement therapywith estrogen (E-2) or E-2 plus progesterone in postmenopausal women decreases the age-associated risk of cardiovascular disease by 30-50%. Treatmentof vascular smooth muscle (VSM) cells with physiological concentrations ofE-2 has been shown to inhibit growth factor-stimulated cell proliferation. In this study, we tested the hypothesis that E-2 inhibits the age-associated increase in VSM cell proliferation by inhibiting nuclear factor (NF)-kappaB pathway. We investigated the effects of E2 treatment and adenovirus-mediated estrogen receptor (ER)-alpha gene transfer on cell proliferation and NF-kappaB activation using VSM cells cultured from 3-mo-old and 24-mo-old Fischer 344 female rats. Our results demonstrate that VSM cell proliferationwas significantly increased (P< 0.05) in aged compared with young adult female rats. Treatment of VSM cells with physiological concentrations of E-2 inhibited VSM cell proliferation, and this inhibition was significantly greater (P< 0.05) in cells from aged female rats compared with young adults. The inhibitory effects of E-2 on cell proliferation in aged female rats weresignificantly potentiated by overexpression of the human ER-alpha gene into VSM cells. Constitutive and interleukin (IL)-1 beta -stimulated NF-kappaBactivation was significantly greater (P< 0.05) in VSM cells from aged compared with young female rats. E-2 treatment of VSM cells from aged female rats inhibited both constitutive and IL-1<beta>-stimulated NF-kappaB activation. ER-alpha gene transfer into VSM cells from aged female rats further augmented the inhibitory effects of E-2. In conclusion, our data demonstrate that constitutive and IL-1 beta -stimulated NF-kappaB activation is increased in VSM cells from aged female rats due to loss of E-2 and this can be restored back to normal levels by ER-alpha gene transfer and E-2 treatment. Inaddition, increased NF-kappaB signaling may be responsible for increased incidence of cardiovascular disease in postmenopausal females.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 23:26:21