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Titolo:
Influence of leptin and insulin on lipid transfer proteins in human hepatoma cell line, HepG2
Autore:
Kaser, S; Foger, B; Ebenbichler, CF; Kirchmair, R; Gander, R; Ritsch, A; Sandhofer, A; Patsch, JR;
Indirizzi:
Innsbruck Univ, Dept Med, A-6020 Innsbruck, Austria Innsbruck Univ Innsbruck Austria A-6020 t Med, A-6020 Innsbruck, Austria
Titolo Testata:
INTERNATIONAL JOURNAL OF OBESITY
fascicolo: 11, volume: 25, anno: 2001,
pagine: 1633 - 1639
SICI:
0307-0565(200111)25:11<1633:IOLAIO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHOLIPID TRANSFER PROTEIN; HIGH-DENSITY-LIPOPROTEIN; CHOLESTERYL-ESTER TRANSFER; CORONARY HEART-DISEASE; ADENOVIRUS-MEDIATED OVEREXPRESSION; DEPENDENT DIABETES-MELLITUS; HDL CHOLESTEROL; ADIPOSE-TISSUE; MESSENGER-RNA; ACUTE HYPERINSULINEMIA;
Keywords:
leptin; insulin; hepatic secretion; phospholipid transfer protein; cholesteryl ester transfer protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Patsch, JR Innsbruck Univ, Dept Med, Anichstr 35, A-6020 Innsbruck, Austria Innsbruck Univ Anichstr 35 Innsbruck Austria A-6020 k, Austria
Citazione:
S. Kaser et al., "Influence of leptin and insulin on lipid transfer proteins in human hepatoma cell line, HepG2", INT J OBES, 25(11), 2001, pp. 1633-1639

Abstract

AIM: Phospholipid transfer protein (PLTP) and cholesteryl ester transfer protein (CETP) are key enzymes in lipoprotein metabolism facilitating the transfer and exchange of cholesteryl esters, triglycerides and phospholipids between lipoproteins. In the study presented here, we investigated the influence of two hormones-the adipocyte-derived hormone leptin as well as insulin on the hepatic secretion of both, PLTP and CETPMETHODS: PLTP activity and CETP concentration-measured by exogenous substrate assay and enzyme-linked immunosorbent assay-were determined in supernatant of human hepatoma cell line HepG2 after single or combined exposure to leptin and insulin at physiological and supraphysiological concentrations, respectively. Messenger-RNA of PLTP and CETP was quantified by Northern blot analysis. RESULTS: Leptin suppressed PLTP activity and CETP-concentration by up to 33% and 23%, respectively. Insulin also suppressed PLTP activity by up to 11% and CETP-concentration by up to 16%. In combination, the two hormones hadadditive suppressive effects for both, PLTP activity and CETP-concentration. Northern blot analysis showed no difference in m-RNA levels after exposure to leptin or insulin. CONCLUSIONS: Leptin and insulin, both known to increase with body fat mass, suppress production of PLTP and CETP in HepG2 cells, When extrapolated tothe in vivo situation, this suppressive effect may constitute a mechanism counteracting the potentially harmful action of lipid transfer proteins, particularly reduction of HDL-cholesterol, in conditions frequently associated with increased plasma triglyceride levels such as obesity and insulin resistance.

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Documento generato il 04/07/20 alle ore 20:46:00